Home / Carboxypeptidase A1/CPA1 / Mouse Carboxypeptidase A1/CPA1 Antibody

Mouse Carboxypeptidase A1/CPA1 Antibody

Catalog #: AF2765
23 Citations Datasheet / COA / SDS
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AF2765
AF2765-SP
Product Details
Citations (23)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Carboxypeptidase A1/CPA1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CPA1 is observed and approximately 10% cross-reactivity with rhCPA2, rhCPB1, and recombinant mouse CPA4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carboxypeptidase A1/CPA1
Asn17-Tyr419
Accession # Q7TPZ8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Carboxypeptidase A1/CPA1 (Catalog # 2765-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Carboxypeptidase A1/CPA1 (Catalog # 2765-ZN), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Mouse Carboxypeptidase A1/CPA1 (0.25 µg/mL, Catalog # 2765‑ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM). The Neutralization Dose (ND50) is typically 2 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase A1/CPA1

Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of mouse CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified recombinant CPA1 corresponds to the pro form, which can be activated and assayed.

References
  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 812  Academic Press, San Diego.
Entrez Gene IDs
1357 (Human); 109697 (Mouse)
Alternate Names
carboxypeptidase A1 (pancreatic); Carboxypeptidase A1; CPA; CPA1; EC 3.4.17; EC 3.4.17.1

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Citations for Mouse Carboxypeptidase A1/CPA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Deletion of Histone Methyltransferase G9a Suppresses Mutant Kras-driven Pancreatic Carcinogenesis
    Authors: Kato H, Tateishi K, Fujiwara H et al.
    Cancer Genomics Proteomics
  2. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster
    Authors: Miguel-Escalada I, Maestro MA, Balboa D et al.
    Developmental cell
  3. kappa B-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis
    Authors: Beel S, Kolloch L, Apken LH et al.
    Nat Commun
  4. 5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma
    Authors: Ebelt ND, Zuniga E, Johnson BL et al.
    Front Immunol
  5. Identification of Novel Ligands for Targeted Antifibrotic Therapy of Chronic Pancreatitis
    Authors: J Hung, R Awasthi, AL Klibanov, KA Kelly
    International Journal of Nanomedicine, 2021-08-14;16(0):5495-5512.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Tracing oncogene-driven remodelling of the intestinal stem cell niche
    Authors: Min Kyu Yum, Seungmin Han, Juergen Fink, Szu-Hsien Sam Wu, Catherine Dabrowska, Teodora Trendafilova et al.
    Nature
  7. Deoxyhypusine synthase, an essential enzyme for hypusine biosynthesis, is required for proper exocrine pancreas development
    Authors: Leah R. Padgett, Morgan A. Robertson, Emily K. Anderson‐Baucum, Craig T. Connors, Wenting Wu, Raghavendra G. Mirmira et al.
    The FASEB Journal
  8. A gene-environment-induced epigenetic program initiates tumorigenesis
    Authors: D Alonso-Cur, YJ Ho, C Burdziak, JLV Maag, JP Morris, R Chandwani, HA Chen, KM Tsanov, FM Barriga, W Luan, N Tasdemir, G Livshits, E Azizi, J Chun, JE Wilkinson, L Mazutis, SD Leach, R Koche, D Pe'er, SW Lowe
    Nature, 2021-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. An In Vivo Kras Allelic Series Reveals Distinct Phenotypes of Common Oncogenic Variants
    Authors: Maria Paz Zafra, Marie J. Parsons, Jangkyung Kim, Direna Alonso-Curbelo, Sukanya Goswami, Emma M. Schatoff et al.
    Cancer Discovery
  10. Desmoplasia and oncogene driven acinar-to-ductal metaplasia are concurrent events during acinar cell-derived pancreatic cancer initiation in young adult mice
    Authors: BL Johnson, M d'Alincour, S Mackenzie-, M D'Apuzzo, HP Shih, ER Manuel, DJ Diamond
    PLoS ONE, 2019-09-06;14(9):e0221810.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Prevention and Reversion of Pancreatic Tumorigenesis through a Differentiation-Based Mechanism
    Authors: Nathan M. Krah, Shuba M. Narayanan, Deanne E. Yugawa, Julie A. Straley, Christopher V. E. Wright, Raymond J. MacDonald et al.
    Developmental Cell
  12. The Loss of ATRX Increases Susceptibility to Pancreatic Injury and Oncogenic KRAS in Female But Not Male Mice
    Authors: CC Young, RM Baker, CJ Howlett, T Hryciw, JE Herman, D Higgs, R Gibbons, H Crawford, A Brown, CL Pin
    Cell Mol Gastroenterol Hepatol, 2018-09-14;7(1):93-113.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Arid1a restrains Kras-dependent changes in acinar cell identity
    Authors: Geulah Livshits, Direna Alonso-Curbelo, John P Morris, Richard Koche, Michael Saborowski, John Erby Wilkinson et al.
    eLife
  14. SEC23B is required for pancreatic acinar cell function in adult mice
    Authors: R Khoriaty, N Vogel, MJ Hoenerhoff, MD Sans, G Zhu, L Everett, B Nelson, H Durairaj, B McKnight, B Zhang, SA Ernst, D Ginsburg, JA Williams
    Mol. Biol. Cell, 2017-05-24;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors
    Authors: NAJ Krentz, D van Hoof, Z Li, A Watanabe, M Tang, C Nian, MS German, FC Lynn
    Dev. Cell, 2017-04-24;41(2):129-142.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Loss of ATM accelerates pancreatic cancer formation and epithelial–mesenchymal transition
    Authors: Ronan Russell, Lukas Perkhofer, Stefan Liebau, Qiong Lin, André Lechel, Fenja M Feld et al.
    Nature Communications
  17. The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma
    Authors: Nathan M Krah, Jean-Paul De La O, Galvin H Swift, Chinh Q Hoang, Spencer G Willet, Fong Chen Pan et al.
    eLife
  18. Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
    Authors: Hou Y, Ernst S, Stuenkel E, Lentz S, Williams J
    PLoS ONE, 2015-05-07;10(5):e0125596.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. A modular and flexible ESC-based mouse model of pancreatic cancer.
    Authors: Saborowski, Michael, Saborowski, Anna, Morris, John P 4, Bosbach, Benedikt, Dow, Lukas E, Pelletier, Jerry, Klimstra, David S, Lowe, Scott W
    Genes Dev, 2014-01-01;28(1):85-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Lineage tracing reveals the dynamic contribution of Hes1+ cells to the developing and adult pancreas.
    Authors: Kopinke D, Brailsford M, Shea JE, Leavitt R, Scaife CL, Murtaugh LC
    Development, 2011-02-01;138(3):431-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Deletion of pancreas-specific miR-216a reduces beta-cell mass and inhibits pancreatic cancer progression in mice
    Authors: Erener S, Ellis CE, Ramzy A Et al.
    Cell reports. Medicine
  22. Acetyl-CoA metabolism supports multi-step pancreatic tumorigenesis
    Authors: A Carrer, S Trefely, S Zhao, S Campbell, RJ Norgard, KC Schultz, S Sidoli, JLD Parris, HC Affronti, S Sivanand, S Egolf, Y Sela, M Trizzino, A Gardini, BA Garcia, NW Snyder, BZ Stanger, K Wellen
    Cancer Discov, 2019-01-09;0(0):.
  23. Mitogen-activated Protein Kinase Kinase Activity Maintains Acinar-to-Ductal Metaplasia and Is Required for Organ Regeneration in Pancreatitis
    Authors: CJ Halbrook, HJ Wen, JM Ruggeri, KK Takeuchi, Y Zhang, MP di Maglian, HC Crawford
    Cell Mol Gastroenterol Hepatol, 2017-01-01;3(1):99-118.

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