Mouse Cathepsin E Biotinylated Antibody

Catalog #: BAF1130
1 Citation Datasheet / COA / SDS

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BAF1130

All Cathepsin E Antibodies

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Mouse Cathepsin E Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects pro and mature mouse Cathepsin E in Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant human Cathepsin E is observed and less than 1% cross-reactivity with recombinant mouse Cathepsin D is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin E
Gln19-Pro397
Accession # P70269

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Cathepsin E (Catalog # 1130-AS)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse lung

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1-18), a propeptide (residues 19-59), and a mature chain (residues 60-397) (1).

References

Tatnell, P.J. et al. (1997) FEBS Lett. 408:62.
Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.), p. 33, Academic Press, San Diego.
Tsukuba, T. et al. (2000) Mol. Cells 10:601.
Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.

Entrez Gene IDs

1510 (Human); 13034 (Mouse)

Alternate Names

CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse Cathepsin E Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1