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Mouse Cathepsin H Antibody

Catalog #: MAB1013
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1013
MAB1013-SP

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Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cathepsin H Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cathepsin H in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin H. In Western blots, no cross-reactivity with rmCathepsin A, D, E, X, and Z or recombinant human Cathepsin B, C, L, or S is observed.
Source
Monoclonal Rat IgG2B Clone # 175909
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin H
Glu22-Val333
Accession # Q3UCD6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Cathepsin H (Catalog # 1013-CY)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin H

Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).

References
  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego. 
  2. Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663.
  3. Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35.
  4. Waghray, A. et al. (2002) J. Biol. Chem. 277:11533.
  5. Guncar, G. et al. (1998) Structure 6:51.
  6. Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113.
  7. del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.
Entrez Gene IDs
1512 (Human); 13036 (Mouse)
Alternate Names
ACC-4; ACC-5; aleurain; cathepsin B3; cathepsin BA; Cathepsin H; CPSB; CTSH; DKFZp686B24257; EC 3.4.22; EC 3.4.22.16; MGC1519; minichain; N-benzoylarginine-beta-naphthylamide hydrolase

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Citations for Mouse Cathepsin H Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Increased Expression of Cathepsins and Obesity-Induced Proinflammatory Cytokines in Lacrimal Glands of Male NOD Mouse
    Authors: Xiaodong Li, Kaijin Wu, Maria Edman, Katja Schenke-Layland, Michelle MacVeigh-Aloni, Srikanth Reddy Janga et al.
    Investigative Opthalmology & Visual Science
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Gene expression analysis of the murine model of amyotrophic lateral sclerosis: studies of the Leu126delTT mutation in SOD1.
    Authors: Fukada Y, Yasui K, Kitayama M, Doi K, Nakano T, Watanabe Y, Nakashima K
    Brain Res., 2007-06-04;1160(0):1-10.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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