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Mouse CCL12/MCP-5 Antibody

Catalog #: MAB428
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB428-SP
MAB428-500
MAB428-100
Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody.
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Mouse CCL12/MCP-5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL12/MCP‑5 in ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 63205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL12/MCP-5
Gly23-Gly104
Accession # Q62401.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)

Mouse CCL12/MCP-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL12/MCP‑5 Biotinylated Antibody (Catalog # BAF428)

Standard: Recombinant Mouse CCL12/MCP-5 Protein (Catalog # 428-P5)

Neutralization
Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse CCL12/MCP‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody. View Larger

Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody. Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/ MCP-5 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP-5 Monoclonal Antibody (Catalog # MAB428). The ND50 is typically 0.5-2.5 µg/mL. Maximum neutralization observed was around 80%.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL12/MCP-5

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

References
  1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
  2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.
Entrez Gene IDs
20293 (Mouse)
Alternate Names
C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12

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Citation for Mouse CCL12/MCP-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The extracellular matrix protein fibulin-3/EFEMP1 promotes pleural mesothelioma growth by activation of PI3K/Akt signaling
    Authors: Arivazhagan Roshini, Chandra Goparaju, Somanath Kundu, Mohan S. Nandhu, Sharon L. Longo, John A. Longo et al.
    Frontiers in Oncology

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