Mouse CCL12/MCP-5 Biotinylated Antibody

Catalog #: BAF428
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF428

All CCL12/MCP-5 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL12/MCP-5 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL12/MCP-5 in ELISAs and Western blots. In sandwich immunoassays, less than 0.07% cross-reactivity with recombinant human (rh) MCP‑1, rhMCP-2, rhMCP-3, rhMCP-4, recombinant mouse (rm) MIP‑1 gamma, rmJE, and rmMARC is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CCL12/MCP-5 (R&D Systems, Catalog # 428-P5)
Gly23-Gly104
Accession # Q62401

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)

Mouse CCL12/MCP-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse CCL12/MCP‑5 Antibody (Catalog # MAB428)

Standard: Recombinant Mouse CCL12/MCP-5 Protein (Catalog # 428-P5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL12/MCP-5

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid sequence identity in the mature protein). Mouse CCL12 encodes a 104 amino acid residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3 or CCR5.

References

Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.

Entrez Gene IDs

20293 (Mouse)

Alternate Names

C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse CCL12/MCP-5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1