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Mouse CCL20/MIP-3 alpha Antibody

Catalog #: MAB7601
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7601-SP
MAB7601-100
MAB7601-500
Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody.
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Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL20/MIP-3 alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL20/MIP-3 alpha in ELISAs. Does not cross-react with recombinant human (rh) CCL19, rhCCL20, recombinant mouse (rm) CCL3, 4, 9, 19, rmCXCL2, or recombinant rat CCL20.
Source
Monoclonal Rat IgG1 Clone # 114908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala28-Met97
Accession # O89093
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse CCL20/MIP-3 alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog # BAF760)

Standard: Recombinant Mouse CCL20/MIP-3 alpha Protein (Catalog # 760-M3)

Neutralization
Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL20/MIP‑3 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody. View Larger

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody. Recombinant Mouse CCL20/MIP-3a (Catalog # 760-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP-3a (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB7601). The ND50 is typically 3-15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 amino acid (aa) residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71% and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

References
  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.
Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Citations for Mouse CCL20/MIP-3 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils
    Authors: Bénard, A;Mittelstädt, A;Klösch, B;Glanz, K;Müller, J;Schoen, J;Nüse, B;Brunner, M;Naschberger, E;Stürzl, M;Mattner, J;Muñoz, LE;Sohn, K;Grützmann, R;Weber, GF;
    Cell reports
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  2. Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses
    Authors: T Hirz, S Mei, H Sarkar, Y Kfoury, S Wu, BM Verhoeven, AO Subtelny, DV Zlatev, MW Wszolek, K Salari, E Murray, F Chen, EZ Macosko, CL Wu, DT Scadden, DM Dahl, N Baryawno, PJ Saylor, PV Kharchenko, DB Sykes
    Nature Communications, 2023-02-07;14(1):663.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Recruitment and activation of type 3 innate lymphoid cells promote antitumor immune responses
    Authors: M Bruchard, M Geindreau, A Perrichet, C Truntzer, E Ballot, R Boidot, C Racoeur, E Barsac, F Chalmin, C Hibos, T Baranek, C Paget, B Ryffel, C Rébé, C Paul, F Végran, F Ghiringhel
    Nature Immunology, 2022-01-31;23(2):262-274.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Keratinocyte transglutaminase 2 promotes CCR6+ &gamma&deltaT-cell recruitment by upregulating CCL20 in psoriatic inflammation
    Authors: JW Shin, MA Kwon, J Hwang, SJ Lee, JH Lee, HJ Kim, KB Lee, SJ Lee, EM Jeong, JH Chung, IG Kim
    Cell Death Dis, 2020-04-30;11(4):301.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. PTH induces bone loss via microbial-dependent expansion of intestinal TNF+ T cells and Th17 cells
    Authors: M Yu, A Malik Tyag, JY Li, J Adams, TL Denning, MN Weitzmann, RM Jones, R Pacifici
    Nat Commun, 2020-01-24;11(1):468.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  6. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  7. Autoimmune Renal Disease Is Exacerbated by S1P-Receptor-1-Dependent Intestinal Th17 Cell Migration to the Kidney
    Authors: Ulf Panzer
    Immunity, 2016-11-15;45(5):1078-1092.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  8. The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages.
    Authors: Boyle S, Faulkner J, McColl S, Kochetkova M
    Mol Cancer, 2015-06-06;14(0):115.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  9. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
    Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
    J Invest Dermatol, 2012-08-16;133(1):164-71.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Tc17 Cells Mediate Vaccine Immunity against Lethal Fungal Pneumonia in Immune Deficient Hosts Lacking CD4+ T Cells.
    Authors: Nanjappa SG, Heninger E, Wuthrich M, Gasper DJ, Klein BS
    PLoS Pathog., 2012-07-19;8(7):e1002771.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. CCL20/CCR6 blockade enhances immunity to RSV by impairing recruitment of DC.
    Authors: Kallal LE, Schaller MA, Lindell DM, Lira SA, Lukacs NW
    Eur. J. Immunol., 2010-04-01;40(4):1042-52.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection.
    Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR
    J. Immunol., 2009-03-01;182(5):3055-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. The chemokine receptor CCR6 is an important component of the innate immune response.
    Authors: Wen H, Hogaboam CM, Lukacs NW, Cook DN, Lira SA, Kunkel SL
    Eur. J. Immunol., 2007-09-01;37(9):2487-98.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Ovariectomy induces bone loss via microbial-dependent trafficking of intestinal TNF+ T cells and Th17 cells
    Authors: Mingcan Yu, Subhashis Pal, Cameron W. Paterson, Jau-Yi Li, Abdul Malik Tyagi, Jonathan Adams et al.
    Journal of Clinical Investigation

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