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Mouse CCL22/MDC ELISA Kit - Quantikine

Catalog #: MCC220
23 Citations 1 Review Datasheet / COA / SDS
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MCC220
Mouse CCL22/MDC ELISA Standard Curve
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Product Details
Procedure
Citations (23)
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Supplemental Products
Reviews (1)
Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse CCL22/MDC Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL)
Sensitivity
1.8 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse MDC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse MDC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MDC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MDC and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MDC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MDC.

Recovery

The recovery of mouse MDC spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 102 87-112
EDTA Plasma (n=4) 94 89-98
Heparin Plasma (n=4) 90 80-109
Serum (n=5) 91 82-100

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MDC in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CCL22/MDC ELISA Linearity

Scientific Data

N/A Mouse CCL22/MDC ELISA Standard Curve View Larger

Mouse CCL22/MDC ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL22/MDC

Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.

Entrez Gene IDs:
6367 (Human); 20299 (Mouse)
Alternate Names:
A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse CCL22/MDC Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization
    Authors: K Ming-Chin, AA Achuthan, DP De Souza, TJ Lupancu, KJ Binger, MKS Lee, Y Xu, MJ McConville, NA de Weerd, D Dragoljevi, PJ Hertzog, AJ Murphy, JA Hamilton, AJ Fleetwood
    Cell Reports, 2022-04-19;39(3):110719.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22
    Authors: A Jorapur, LA Marshall, S Jacobson, M Xu, S Marubayash, M Zibinsky, DX Hu, O Robles, JJ Jackson, V Baloche, P Busson, D Wustrow, DG Brockstedt, O Talay, PD Kassner, G Cutler
    PloS Pathogens, 2022-01-13;18(1):e1010200.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Mechanism of chlorogenic acid in alveolar macrophage polarization in Klebsiella pneumoniae-induced pneumonia
    Authors: QR Li, SR Tan, L Yang, W He, L Chen, FX Shen, Z Wang, HF Wang
    Journal of leukocyte biology, 2021-09-29;0(0):.
    Species: Mouse
    Sample Types: BALF
  4. Isoalantolactone alleviates ovalbumin?induced asthmatic inflammation by reducing alternatively activated macrophage and STAT6/PPAR?gamma/KLF4 signals
    Authors: Y Song, X Li, F Liu, H Zhu, Y Shen
    Molecular Medicine Reports, 2021-08-09;24(4):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Neutralization of IL-33 modifies the type 2 and type 3 inflammatory signature of viral induced asthma exacerbation
    Authors: KJ Warren, JA Poole, JM Sweeter, JM DeVasure, JD Dickinson, RS Peebles, TA Wyatt
    Respiratory Research, 2021-07-15;22(1):206.
    Species: Mouse
    Sample Types: BALF
  6. A transcription factor PU.1 is critical for Ccl22 gene expression in dendritic cells and macrophages
    Authors: T Yashiro, S Nakano, K Nomura, Y Uchida, K Kasakura, C Nishiyama
    Sci Rep, 2019-02-04;9(1):1161.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge
    Authors: S Yi, J Zhai, R Niu, G Zhu, M Wang, J Liu, H Huang, Y Wang, X Jing, L Kang, W Song, Y Shi, H Tang
    Nat Commun, 2018-09-24;9(1):3879.
    Species: Mouse
    Sample Types: BALF
  8. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality
    Authors: IK Kim, K Kim, E Lee, DS Oh, CS Park, S Park, JM Yang, JH Kim, HS Kim, DT Shima, JH Kim, SH Hong, YH Cho, YH Kim, JB Park, GY Koh, YS Ju, HK Lee, S Lee, I Kim
    J. Exp. Med., 2018-02-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  9. Airway inflammation after epicutaneous sensitization of mice requires protease activity of low-dose allergen inhalation
    Authors: I Nishioka, T Takai, N Maruyama, S Kamijo, P Suchiva, M Suzuki, S Kunimine, H Ochi, S Shimura, K Sudo, H Ogawa, K Okumura, S Ikeda
    J. Allergy Clin. Immunol., 2018-02-06;0(0):.
    Species: Mouse
    Sample Types: BALF
  10. CCL11 promotes migration and proliferation of mouse neural progenitor cells
    Authors: F Wang, N Baba, Y Shen, T Yamashita, E Tsuru, M Tsuda, N Maeda, Y Sagara
    Stem Cell Res Ther, 2017-02-07;8(1):26.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015-02-18;6(0):6224.
    Species: Mouse
    Sample Types: BALF
  12. Mycoplasma pneumoniae CARDS toxin exacerbates ovalbumin-induced asthma-like inflammation in BALB/c mice.
    Authors: Medina, Jorge L, Coalson, Jacqueli, Brooks, Edward G, Le Saux, Claude J, Winter, Vicki T, Chaparro, Adriana, Principe, Molly F, Solis, Laura, Kannan, T R, Baseman, Joel B, Dube, Peter H
    PLoS ONE, 2014-07-24;9(7):e102613.
    Species: Mouse
    Sample Types: BALF
  13. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.
    Authors: Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M
    J Immunol, 2014-01-17;192(4):1671-80.
    Species: Mouse
    Sample Types: Cell Lysates
  14. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
    J. Immunol., 2012-07-30;189(5):2545-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets.
    Authors: Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB
    J. Clin. Invest., 2011-08-01;121(0):3024.
    Species: Mouse
    Sample Types: Cell Lysates
  16. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2011-05-10;179(1):302-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.
    Authors: Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C
    J. Immunol., 2011-01-10;186(4):2372-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation.
    Authors: Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD
    J. Immunol., 2009-01-01;182(1):623-35.
    Species: Mouse
    Sample Types: BALF
  19. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
    Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
    J. Exp. Med., 2008-08-11;205(9):2075-84.
    Species: Mouse
    Sample Types: Serum
  20. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
    Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
    Am. J. Pathol., 2008-04-10;172(5):1209-21.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
    Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
    J. Exp. Med., 2008-03-03;205(3):699-710.
    Species: Mouse
    Sample Types: BALF
  22. iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity.
    Authors: Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT
    J. Immunol., 2007-10-01;179(7):4661-71.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
    Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
    Proc. Natl. Acad. Sci. U.S.A., 2007-02-05;104(7):2319-24.
    Species: Mouse
    Sample Types: Serum

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Mouse CCL22/MDC Quantikine ELISA Kit
By Anonymous on 12/07/2017
Sample Tested: Plasma
Mouse CCL22/MDC Quantikine ELISA Kit MCC220