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Mouse CCL25/TECK Antibody

Catalog #: MAB481
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB481-SP
MAB481-100
MAB481-500
Chemotaxis Induced by CCL25/TECK and Neutralization by Mouse CCL25/TECK Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL25/TECK Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse (rm) CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22 or 24, recombinant human (rh) CCL2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26, or recombinant rat (rr) CCL20 is observed.
Source
Monoclonal Rat IgG2B Clone # 89818
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL25/TECK
Gln24-Asn144
Accession # O35903.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL25/TECK (Catalog # 481-TK)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR9. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 1.6 µg/mL Recombinant Mouse CCL25/TECK.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL25/TECK and Neutralization by Mouse CCL25/TECK Antibody. View Larger

Chemotaxis Induced by CCL25/TECK and Neutralization by Mouse CCL25/TECK Antibody. Recombinant Mouse CCL25/TECK (Catalog # 481-TK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL25/TECK (1.6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL25/TECK Monoclonal Antibody (Catalog # MAB481). The ND50 is typically 1.5 - 6.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL25/TECK

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein.  Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant mouse CCL25 has been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes (1).

References
  1. Vicari, A.P. et al. (1997) Immunity 7:291.
Entrez Gene IDs
6370 (Human); 20300 (Mouse)
Alternate Names
C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine

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Citations for Mouse CCL25/TECK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Inflammation triggers ILC3 patrolling of the intestinal barrier
    Authors: A Jarade, Z Garcia, S Marie, A Demera, I Prinz, P Bousso, JP Di Santo, N Serafini
    Nature Immunology, 2022-08-23;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012-05-01;42(5):1250-60.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. A subset of interleukin-21+ chemokine receptor CCR9+ T helper cells target accessory organs of the digestive system in autoimmunity.
    Authors: McGuire HM, Vogelzang A, Ma CS, Hughes WE, Silveira PA, Tangye SG, Christ D, Fulcher D, Falcone M, King C
    Immunity, 2011-04-22;34(4):602-15.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization.
    Authors: Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y
    Blood, 2006-06-29;108(8):2531-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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