Mouse CCL27/CTACK Biotinylated Antibody

Catalog #: BAM7251 Datasheet / COA / SDS

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BAM7251

All CCL27/CTACK Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL27/CTACK Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL27/CTACK in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 68706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CCL27/CTACK
Leu26-Asn120
Accession # NP_035466

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CCL27/CTACK (Catalog # 725-CK)

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of mouse skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL27/CTACK

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively (2). It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin (4-7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7-9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10-12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA⁺ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13-15).

References

Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 258:737.
Gortz, A. et al. (2002) J. Immunol. 169:1387.
Morales, J. et al. (1999) Proc. Natl. Acad. Sci. USA 96:14470.
Homey, B. et al. (2000) J. Immunol. 164:3465.
Homey, B. et al. (2002) Nat. Med. 8:157.
Inokuma, D. et al. (2006) Stem Cells 24:2810.
Jarmin, D. et al. (2000) J. Immunol. 164:3460.
Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
Soler, D. et al. (2003) Blood 101:1677.
Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

Entrez Gene IDs

10850 (Human); 20301 (Mouse); 362505 (Rat)

Alternate Names

ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27