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Mouse CCL28 Antibody

Catalog #: MAB533
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB533-SP
MAB533-500
MAB533-100
Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL28 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL28 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) CCL28 is observed and no cross-reactivity with rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10, 11, 12, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.
Source
Monoclonal Rat IgG2B Clone # 134306
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL28 (Catalog # 533-VI) under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND50) is typically 2.5-5.0 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL28.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody. View Larger

Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody. Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL28 Monoclonal Antibody (Catalog # MAB533). The ND50 is typically 2.5-5.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL28

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.

References
  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Entrez Gene IDs
56477 (Human); 56838 (Mouse)
Alternate Names
CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

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Citations for Mouse CCL28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Chromatin accessibility uncovers KRAS-driven FOSL2 promoting pancreatic ductal adenocarcinoma progression through up-regulation of CCL28
    Authors: Zhang, S;Li, P;Li, J;Gao, J;Qi, Q;Dong, G;Liu, X;Jiao, Q;Wang, Y;Du, L;Zhan, H;Xu, S;Wang, C;
    British journal of cancer
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Bile salt hydrolase in non-enterotoxigenic Bacteroides potentiates colorectal cancer
    Authors: L Sun, Y Zhang, J Cai, B Rimal, ER Rocha, JP Coleman, C Zhang, RG Nichols, Y Luo, B Kim, Y Chen, KW Krausz, CC Harris, AD Patterson, Z Zhang, S Takahashi, FJ Gonzalez
    Nature Communications, 2023-02-10;14(1):755.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019-09-26;11(18):7402-7415.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. POL7085 or anti-CCL28 treatment inhibits development of post-paramyxoviral airway disease
    Authors: BJ Buelow, M Rohlfing, F Jung, GJ Douglas, MH Grayson
    Immun Inflamm Dis, 2017-02-02;5(2):98-108.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Structure-function analysis of CCL28 in the development of post-viral asthma.
    Authors: Thomas M, Buelow B, Nevins A, Jones S, Peterson F, Gundry R, Grayson M, Volkman B
    J Biol Chem, 2015-01-02;290(7):4528-36.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control.
    Authors: Cha HR, Ko HJ, Kim ED
    J. Immunol., 2011-08-10;187(6):3044-52.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011-07-13;475(7355):226-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
    Authors: Mosconi E, Rekima A, Seitz-Polski B
    Mucosal Immunol, 2010-05-19;3(5):461-74.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo.
    Authors: Kutzler MA, Kraynyak KA, Nagle SJ, Parkinson RM, Zharikova D, Chattergoon M, Maguire H, Muthumani K, Ugen K, Weiner DB
    Gene Ther., 2009-10-22;17(1):72-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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