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Mouse CCL28 Biotinylated Antibody

Catalog #: BAF533
1 Citation Datasheet / COA / SDS
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BAF533
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL28 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL28 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human CCL28 and recombinant mouse CTACK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL28 (Catalog # 533-VI)

Mouse CCL28 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CCL28 Antibody (Catalog # MAB5331)

Standard: Recombinant Mouse CCL28 Protein (Catalog # 533-VI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL28

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.

References
  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Entrez Gene IDs
56477 (Human); 56838 (Mouse)
Alternate Names
CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

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Citation for Mouse CCL28 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CCL28-Deficient Mice Have Reduced IgA Antibody-Secreting Cells and an Altered Microbiota in the Colon
    Authors: K Matsuo, D Nagakubo, S Yamamoto, A Shigeta, S Tomida, M Fujita, T Hirata, I Tsunoda, T Nakayama, O Yoshie
    J. Immunol., 2017-12-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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