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Mouse CCL3/MIP-1 alpha Antibody

Catalog #: MAB450
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB450-SP
MAB450-500
MAB450-100
Product Details
Citations (14)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL3/MIP-1 alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/
MIP‑1 beta  is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed.
Source
Monoclonal Rat IgG2A Clone # 39624
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL3/MIP-1 alpha
Ala24-Ala92
Accession # P10855
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL3/MIP-1 alpha Isoform LD78a (Catalog # 450-MA) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines.

Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.

References
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Entrez Gene IDs
6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names
C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

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Citations for Mouse CCL3/MIP-1 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Increased generation of HIV-1 gp120-reactive CD8+ T cells by a DNA vaccine construct encoding the chemokine CCL3.
    Authors: Oynebraten I, Hinkula J, Fredriksen A, Bogen B
    PLoS ONE, 2014-08-14;9(8):e104814.
  2. Epigenetic scarring of exhausted T cells hinders memory differentiation upon eliminating chronic antigenic stimulation
    Authors: MS Abdel-Hake, S Manne, JC Beltra, E Stelekati, Z Chen, K Nzingha, MA Ali, JL Johnson, JR Giles, D Mathew, AR Greenplate, G Vahedi, EJ Wherry
    Nature Immunology, 2021-07-26;22(8):1008-1019.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. A DNA Vaccine That Encodes an Antigen-Presenting Cell-Specific Heterodimeric Protein Protects against Cancer and Influenza
    Authors: R Braathen, HCL Spång, DM Hinke, J Blazevski, S Bobic, E Fossum, B Bogen
    Mol Ther Methods Clin Dev, 2020-01-25;17(0):378-392.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  4. Cellular content plays a crucial role in Non-typeable Haemophilus influenzae infection of preinflamed Junbo mouse middle ear
    Authors: Pratik P. Vikhe, Tom Purnell, Steve D.M. Brown, Derek W. Hood
    Cellular Microbiology
  5. Inhibition of CCL3 abrogated precursor cell fusion and bone erosions in human osteoclast cultures and murine collagen-induced arthritis
    Authors: Lauren A Jordan, Malin C Erlandsson, Benjamin F Fenner, Ruth Davies, Ann K Harvey, Ernest H Choy et al.
    Rheumatology (Oxford)
  6. Induction of the MCP chemokine cluster cascade in the periphery by cancer cell-derived CCL3
    Authors: Elena Farmaki
    Cancer Lett, 2016-12-29;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates, In Vivo
    Applications: Neutralization
  7. Immune signatures of protective spleen memory CD8 T cells
    Sci Rep, 2016-11-24;6(0):37651.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Mechanisms of Invariant NKT Cell Activity in Restraining Bacillus anthracis Systemic Dissemination
    J Immunol, 2016-09-07;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma.
    Authors: Deepak P, Kumar S, Kishore D, Acharya A
    Int. Immunol., 2009-11-30;22(1):53-63.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  11. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Mosmann T
    Eur. J. Immunol., 2004-06-01;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
    Authors: Reading PC, Symons JA, Smith GL
    J. Immunol., 2003-02-01;170(3):1435-42.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  14. Regulatory effects of eotaxin on acute lung inflammatory injury.
    Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
    J. Immunol., 2001-04-15;166(8):5208-18.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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