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Mouse CCL3/MIP-1 alpha PE-conjugated Antibody

Catalog #: IC450P
8 Citations Datasheet / COA / SDS
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IC450P
Detection of CCL3/MIP‑1 alpha in LPS-stimulated RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (8)
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Mouse CCL3/MIP-1 alpha PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/MIP‑1 beta  is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed. Neutralizes the biological activity of recombinant mouse CCL3 and will not block the biological activity of recombinant human CCL3.
Source
Monoclonal Rat IgG2A Clone # 39624
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL3/MIP-1 alpha
Ala24-Ala92
Accession # P10855
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CCL3/MIP-1a antibody in LPS-stimulated RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CCL3/MIP‑1 alpha in LPS-stimulated RAW 264.7 Mouse Cell Line by Flow Cytometry. LPS-stimulated RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCL3/MIP-1a PE-conjugated Monoclonal Antibody (Catalog # IC450P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CCL3/MIP-1 alpha

The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines.

Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.

References
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Entrez Gene IDs
6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names
C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

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Citations for Mouse CCL3/MIP-1 alpha PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer.
    Authors: Zeyu Chen, Eri Arai, Omar Khan, Zhen Zhang, Shin Foong Ngiow, Yuan He et al.
    Cell
  2. Epigenetic scarring of exhausted T cells hinders memory differentiation upon eliminating chronic antigenic stimulation
    Authors: MS Abdel-Hake, S Manne, JC Beltra, E Stelekati, Z Chen, K Nzingha, MA Ali, JL Johnson, JR Giles, D Mathew, AR Greenplate, G Vahedi, EJ Wherry
    Nature Immunology, 2021-07-26;22(8):1008-1019.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Novel malaria antigen Plasmodium yoelii E140 induces antibody-mediated sterile protection in mice against malaria challenge
    Authors: EC Smith, KJ Limbach, N Rangel, K Oda, JS Bolton, M Du, K Gowda, J Wang, JK Moch, S Sonawane, R Velasco, A Belmonte, R Danner, JM Lumsden, NB Patterson, M Sedegah, MR Hollingdal, TL Richie, JB Sacci, ED Villasante, JC Aguiar
    PLoS ONE, 2020-05-14;15(5):e0232234.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Long-Term Persistence of Exhausted CD8�T Cells in Chronic Infection Is Regulated by MicroRNA-155
    Authors: E Stelekati, Z Chen, S Manne, M Kurachi, MA Ali, K Lewy, Z Cai, K Nzingha, LM McLane, JL Hope, AJ Fike, PD Katsikis, EJ Wherry
    Cell Rep, 2018-05-15;23(7):2142-2156.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. miR-150 Regulates Memory CD8�T Cell Differentiation via c-Myb
    Authors: Z Chen, E Stelekati, M Kurachi, S Yu, Z Cai, S Manne, O Khan, X Yang, EJ Wherry
    Cell Rep, 2017-09-12;20(11):2584-2597.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Prime-Boost Immunization Eliminates Metastatic Colorectal Cancer by Producing High-Avidity Effector CD8(+) T Cells
    Authors: B Xiang, TR Baybutt, L Berman-Boo, MS Magee, SA Waldman, VY Alexeev, AE Snook
    J. Immunol, 2017-03-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. GUCY2C-directed CAR-T cells oppose colorectal cancer metastases without autoimmunity
    Authors: Adam E Snook
    Oncoimmunology, 2016-09-02;5(10):e1227897.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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