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Mouse CCR10 Antibody

Catalog #: MAB2815
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2815
MAB2815-SP
Detection of CCR10 in HEK293 Human Cell Line Transfected with Mouse CCR10 and eGFP by Flow Cytometry.
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Product Details
Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 248918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C6 rat glioma cell line transfected with mouse CCR10
Met1-Asn362
Accession # NP_031747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR10 antibody in HEK293 Human Cell Line Transfected with Mouse CCR10 and eGFP antibody by Flow Cytometry. View Larger

Detection of CCR10 in HEK293 Human Cell Line Transfected with Mouse CCR10 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse CCR10 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse CCR10 Monoclonal Antibody (Catalog # MAB2815) or Rat IgG2AIsotype Control (Catalog # MAB006, data not shown) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CCR10 in Raw264.7 cells by Flow Cytometry. Raw264.7 cells were stained with Rat Anti-Mouse CCR10 Monoclonal Antibody (Catalog # MAB2815, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs
2826 (Human); 12777 (Mouse); 363682 (Rat)
Alternate Names
CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2

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Citations for Mouse CCR10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Ligand-competent fractalkine receptor is expressed on exosomes
    Authors: EJ Park, PK Myint, MG Appiah, P Worawattan, J Inprasit, O Prajuabjin, ZY Soe, A Gaowa, E Kawamoto, M Shimaoka
    Biochemistry and Biophysics Reports, 2021-02-02;26(0):100932.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019-09-26;11(18):7402-7415.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model
    Authors: NM Bath, X Ding, NA Wilson, BM Verhoven, BA Boldt, A Sukhwal, SR Reese, SE Panzer, A Djamali, RR Redfield
    PLoS ONE, 2019-02-08;14(2):e0211865.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
    Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
    J. Immunol, 2016-02-05;196(6):2514-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Interferon-gamma Promotes Inflammation and Development of T-Cell Lymphoma in HTLV-1 bZIP Factor Transgenic Mice.
    Authors: Mitagami Y, Yasunaga J, Kinosada H, Ohshima K, Matsuoka M
    PLoS Pathog, 2015-08-21;11(8):e1005120.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011-07-13;475(7355):226-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Thymic self-reactivity selects natural interleukin 17-producing T cells that can regulate peripheral inflammation.
    Authors: Marks BR, Nowyhed HN, Choi JY, Poholek AC, Odegard JM, Flavell RA, Craft J
    Nat. Immunol., 2009-09-06;10(10):1125-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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