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Mouse CCR10 PE-conjugated Antibody

Catalog #: FAB2815P
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2815P-025
FAB2815P-100
Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR10 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 248918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C6 rat glioma cell line transfected with mouse CCR10
Met1-Asn362
Accession # NP_031747
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
B16‑F1 mouse melanoma cell line and BaF3 mouse pro-B cell line transfected with mouse CCR10 and eGFP; HEK293 cell line transfected with mCCR10 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR10 antibody in B16-F1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse CCR10 PE-conjugated Monoclonal Antibody (Catalog # FAB2815P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR10 antibody in BaF3 Mouse Cell Line Transfected with CCR10 and eGFP antibody by Flow Cytometry. View Larger

Detection of CCR10 in BaF3 Mouse Cell Line Transfected with CCR10 and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse CCR10 and eGFP was stained with Rat Anti-Mouse CCR10 PE-conjugated Monoclonal Antibody (Catalog # FAB2815P) and. Quadrant markers were set based on control antibody staining (Catalog # IC006P). dView our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CCR10 in HEK293 Human Cell Line transfected with Mouse CCR10 and eGFP vs irrelevant GPCR by Flow Cytometry HEK293 Human Cell Line transfected with Mouse CCR10 and eGFP (A) vs irrelevant GPCR (B) were stained with Rat Anti-Mouse CCR10 PE‑conjugated Monoclonal Antibody (Catalog # fab2815P) and eGFP isotype control antibody (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs
2826 (Human); 12777 (Mouse); 363682 (Rat)
Alternate Names
CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2

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Citations for Mouse CCR10 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Topical application of fucoidan derived from Cladosiphon okamuranus alleviates atopic dermatitis symptoms through immunomodulation
    Authors: BR Chen, KT Hsu, TL Li, YL Chan, CJ Wu
    International immunopharmacology, 2021-11-18;0(0):108362.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Adaptive immunity to murine skin commensals.
    Authors: Shen W, Li W, Hixon J, Bouladoux N, Belkaid Y, Dzutzev A, Durum S
    Proc Natl Acad Sci U S A, 2014-07-07;111(29):E2977-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
    Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
    J. Immunol., 2009-06-01;182(11):6851-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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