Mouse CCR10 PerCP-conjugated Antibody

Catalog #: FAB2815C Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2815C-025
FAB2815C-100

Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry.

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Mouse CCR10 PerCP-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2A Clone # 248918

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

C6 rat glioma cell line transfected with mouse CCR10
Met1-Asn362
Accession # NP_031747

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse CCR10 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2815C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CCR10 in BaF3 Mouse Cell Line Transfected with CCR10 and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse CCR10 and eGFP was stained with Rat Anti-Mouse CCR10 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2815C) and. Quadrant markers were set based on control antibody staining (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs

2826 (Human); 12777 (Mouse); 363682 (Rat)

Alternate Names

CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2