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Mouse CCR2 Antibody

Catalog #: MAB55381
16 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB55381-100
MAB55381-SP
Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry.
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Citations (16)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 475301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
L1.2 mouse pro-B cell line transfected with mouse CCR2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR2 antibody in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP antibody by Flow Cytometry. View Larger

Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with either (A) mouse CCR2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse/Rat CCR2 Monoclonal Antibody (Catalog # MAB55381) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). Quadrant markers were set based on isotype control (Rat IgG2B,Catalog # MAB0061; data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Citations for Mouse CCR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Transitional dendritic cells are distinct from conventional DC2 precursors and mediate proinflammatory antiviral responses
    Authors: Sulczewski, FB;Maqueda-Alfaro, RA;Alcántara-Hernández, M;Perez, OA;Saravanan, S;Yun, TJ;Seong, D;Arroyo Hornero, R;Raquer-McKay, HM;Esteva, E;Lanzar, ZR;Leylek, RA;Adams, NM;Das, A;Rahman, AH;Gottfried-Blackmore, A;Reizis, B;Idoyaga, J;
    Nature immunology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. ZBTB7A regulates MDD-specific chromatin signatures and astrocyte-mediated stress vulnerability in orbitofrontal cortex
    Authors: Fulton, SL;Bendl, J;Gameiro-Ros, I;Fullard, JF;Al-Kachak, A;Lepack, AE;Stewart, AF;Singh, S;Poller, WC;Bastle, RM;Hauberg, ME;Fakira, AK;Chen, M;Cuttoli, RD;Cathomas, F;Ramakrishnan, A;Gleason, K;Shen, L;Tamminga, CA;Milosevic, A;Russo, SJ;Swirski, F;Blitzer, RD;Slesinger, PA;Roussos, P;Maze, I;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Deficiency in CCR2 increases susceptibility of mice to infection with an intracellular pathogen, Francisella tularensis LVS, but does not impair development of protective immunity
    Authors: SL Kurtz, R De Pascali, AI Meierovics, KL Elkins
    PLoS ONE, 2021-03-24;16(3):e0249142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  4. Soluble PD-L1 improved direct ARDS by reducing monocyte-derived macrophages
    Authors: J Xu, J Wang, X Wang, R Tan, X Qi, Z Liu, H Qu, T Pan, Q Zhan, Y Zuo, W Yang, J Liu
    Cell Death Dis, 2020-10-30;11(10):934.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: CyTof
  5. The Angiotensin Receptor Blocker Losartan Suppresses Growth of Pulmonary Metastases via AT1R-Independent Inhibition of CCR2 Signaling and Monocyte Recruitment
    Authors: DP Regan, JW Coy, KK Chahal, L Chow, JN Kurihara, AM Guth, I Kufareva, SW Dow
    J. Immunol., 2019-04-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Proteomic analysis reveals a protective role of specific macrophage subsets in liver repair
    Authors: W Yang, X Zhao, Y Tao, Y Wu, F He, L Tang
    Sci Rep, 2019-02-27;9(1):2953.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. CCR2 enhances CD25 expression by FoxP3+ regulatory T cells and regulates their abundance independently of chemotaxis and CCR2+ myeloid cells
    Authors: Y Zhan, N Wang, A Vasanthaku, Y Zhang, M Chopin, SL Nutt, A Kallies, AM Lew
    Cell. Mol. Immunol., 2018-12-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Self-renewing resident cardiac macrophages limit adverse remodeling following myocardial infarction
    Authors: SA Dick, JA Macklin, S Nejat, A Momen, X Clemente-C, MG Althagafi, J Chen, C Kantores, S Hosseinzad, L Aronoff, A Wong, R Zaman, I Barbu, R Besla, KJ Lavine, B Razani, F Ginhoux, M Husain, MI Cybulsky, CS Robbins, S Epelman
    Nat. Immunol., 2018-12-11;20(1):29-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Effect of CCR2 inhibitor-loaded lipid micelles on inflammatory cell migration and cardiac function after myocardial infarction
    Authors: J Wang, MJ Seo, MB Deci, BR Weil, JM Canty, J Nguyen
    Int J Nanomedicine, 2018-10-15;13(0):6441-6451.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Carbon Monoxide Impairs CD11bLy-6CMonocyte Migration from the Blood to Inflamed Pancreas via Inhibition of the CCL2/CCR2 Axis
    Authors: J Wu, R Zhang, G Hu, HH Zhu, WQ Gao, J Xue
    J. Immunol., 2018-02-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Myeloid-targeted immunotherapies act in synergy to induce inflammation and antitumor immunity
    Authors: CJ Perry, AR Muñoz-Roja, KM Meeth, LN Kellman, RA Amezquita, D Thakral, VY Du, JX Wang, W Damsky, AL Kuhlmann, JW Sher, M Bosenberg, K Miller-Jen, SM Kaech
    J. Exp. Med., 2018-02-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Innate and adaptive lymphocytes sequentially shape the gut microbiota and lipid metabolism
    Authors: K Mao, AP Baptista, S Tamoutouno, L Zhuang, N Bouladoux, AJ Martins, Y Huang, MY Gerner, Y Belkaid, RN Germain
    Nature, 2018-01-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. NK cell heparanase controls tumor invasion and immune surveillance
    Authors: EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth
    J. Clin. Invest., 2017-06-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Antibody blockade of CLEC12A delays EAE onset and attenuates disease severity by impairing myeloid cell CNS infiltration and restoring positive immunity
    Authors: D Sagar, NP Singh, R Ginwala, X Huang, R Philip, M Nagarkatti, P Nagarkatti, K Neumann, J Ruland, AM Andrews, SH Ramirez, ZK Khan, P Jain
    Sci Rep, 2017-06-02;7(1):2707.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Macrophage Death following Influenza Vaccination Initiates the Inflammatory Response that Promotes Dendritic Cell Function in the Draining Lymph Node
    Authors: N Chatziandr, Y Farsakoglu, M Palomino-S, R D'Antuono, DU Pizzagalli, F Sallusto, V Lukacs-Kor, M Uguccioni, D Corti, SJ Turley, A Lanzavecch, MC Carroll, SF Gonzalez
    Cell Rep, 2017-03-07;18(10):2427-2440.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. MAIT cells promote inflammatory monocyte differentiation into dendritic cells during pulmonary intracellular infection
    Authors: Siobhán C Cowley
    J. Exp. Med., 2016-10-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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