Were cells transfected with the complete CCR2 sequence used as an immunogen for Mouse CCR2 antibody, Catalog # MAB55382?

Name: Mouse CCR2 Antibody
Brand: R&D Systems
SKU: MAB55382
Price: 429 USD

Yes. The complete CCR2 sequence was transfected into the L1.2 mouse Pro-B cell line.

Mouse CCR2 Antibody

Catalog #: MAB55382 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB55382-100
MAB55382-SP

1 Image

All CCR2 Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR2 in immunocytochemistry.

Source

Monoclonal Rat IgG_2A Clone # 475303

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

L1.2 mouse pro-B cell line transfected with mouse CCR2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

CCR2 in RAW 264.7 Mouse Cell Line. CCR2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse CCR2 Monoclonal Antibody (Catalog # MAB55382) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Alternate Names

C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

Were cells transfected with the complete CCR2 sequence used as an immunogen for Mouse CCR2 antibody, Catalog # MAB55382?
- Yes. The complete CCR2 sequence was transfected into the L1.2 mouse Pro-B cell line.

View all Antibody FAQs