Mouse CCR2 APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of CCR2 in L1.2 Mouse Cell Line Transfected with Mouse CCR2 by Flow Cytometry. L1.2 mouse pro-B cell line transfected with mouse CCR2 was stained with Rat Anti-Mouse CCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB5538A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB5538A) and Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR2
CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.
Product Datasheets
Citations for Mouse CCR2 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Analysis of the Microglial Sensome
Authors: Suzanne E. Hickman, Joseph El Khoury
Methods Mol. Biol.
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Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes
Authors: Sabine Spath, Florence Roan, Scott R. Presnell, Barbara Höllbacher, Steven F. Ziegler
iScience
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PI3Kδ hyper-activation promotes development of B cells that exacerbate Streptococcus pneumoniae infection in an antibody-independent manner
Authors: Anne-Katrien Stark, Anita Chandra, Krishnendu Chakraborty, Rafeah Alam, Valentina Carbonaro, Jonathan Clark et al.
Nature Communications
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Resident macrophages restrain pathological adipose tissue remodeling and protect vascular integrity in obese mice
Authors: Qi Chen, Si Min Lai, Shaohai Xu, Yingrou Tan, Keith Leong, Dehua Liu et al.
EMBO reports
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Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins
Authors: Lim J, Park H, Heisler J et al.
Elife
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Regulation of age-related macular degeneration-like pathology by complement factor H
Authors: Christopher B. Toomey, Una Kelly, Daniel R. Saban, Catherine Bowes Rickman
Proceedings of the National Academy of Sciences
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CCR2 Signal Facilitates Thymic Egress by Priming Thymocyte Responses to Sphingosine-1-Phosphate
Authors: Abudureyimujiang Aili, Jie Zhang, Jia Wu, Haoming Wu, Xiuyuan Sun, Qihua He et al.
Frontiers in Immunology
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Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints
Authors: M. E. Mikucki, D. T. Fisher, J. Matsuzaki, J. J. Skitzki, N. B. Gaulin, J. B. Muhitch et al.
Nature Communications
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Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer
Authors: Anders Etzerodt, Morgane Moulin, Thomas Koed Doktor, Marcello Delfini, Noushine Mossadegh-Keller, Marc Bajenoff et al.
Journal of Experimental Medicine
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Macrophages promote anti-androgen resistance in prostate cancer bone disease
Authors: Li XF, Selli C, Zhou HL et al.
The Journal of experimental medicine
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Cytokine Deposition Alters Leukocyte Morphology and Initial Recruitment of Monocytes and gamma δT Cells After Corneal Injury
Authors: Sonali Pal-Ghosh, Ahdeah Pajoohesh-Ganji, A. Sue Menko, Hye-young Oh, Gauri Tadvalkar, Daniel R. Saban et al.
Investigative Opthalmology & Visual Science
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Effective-mononuclear cell (E-MNC) therapy alleviates salivary gland damage by suppressing lymphocyte infiltration in Sjögren-like disease
Authors: Kayo Hasegawa, Jorge Luis Montenegro Raudales, Takashi I, Takako Yoshida, Ryo Honma, Mayumi Iwatake et al.
Frontiers in Bioengineering and Biotechnology
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The impact of ranitidine on monocyte responses in the context of solid tumors
Authors: Ava Vila-Leahey, Dakota Rogers, Jean S. Marshall
Oncotarget
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The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
Authors: AE Geller, R Shrestha, MR Woeste, H Guo, X Hu, C Ding, K Andreeva, JH Chariker, M Zhou, D Tieri, CT Watson, RA Mitchell, HG Zhang, Y Li, RCG Martin Ii, EC Rouchka, J Yan
Nature Communications, 2022-02-09;13(1):759.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-20 Cytokines Are Involved in Epithelial Lesions Associated with Virus-Induced COPD Exacerbation in Mice
Authors: M Le Roux, A Ollivier, G Kervoaze, T Beke, L Gillet, M Pichavant, P Gosset
Biomedicines, 2021-12-05;9(12):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Early activation of the cardiac CX3CL1/CX3CR1 axis delays beta-adrenergic-induced heart failure
Authors: M Flamant, N Mougenot, E Balse, L Le Fèvre, F Atassi, EL Gautier, W Le Goff, M Keck, S Nadaud, C Combadière, A Boissonnas, C Pavoine
Scientific Reports, 2021-09-09;11(1):17982.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Inhibition of the canonical Wnt signaling pathway by a beta-catenin/CBP inhibitor prevents heart failure by ameliorating cardiac hypertrophy and fibrosis
Authors: T Methatham, S Tomida, N Kimura, Y Imai, K Aizawa
Scientific Reports, 2021-07-21;11(1):14886.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The CCR2/MCP-1 Chemokine Pathway and Lung Adenocarcinoma
Authors: P Mittal, L Wang, T Akimova, CA Leach, JC Clemente, MR Sender, Y Chen, BJ Turunen, WW Hancock
Cancers, 2020-12-11;12(12):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Macrophage phenotypic switch orchestrates the inflammation and repair/regeneration following acute pancreatitis injury
Authors: J Wu, L Zhang, J Shi, R He, W Yang, A Habtezion, N Niu, P Lu, J Xue
EBioMedicine, 2020-07-30;58(0):102920.
Species: Mouse
Sample Types: Whole Cells
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Low-dose oral cyclophosphamide therapy reduces atherosclerosis progression by decreasing inflammatory cells in a murine model of atherosclerosis
Authors: Y Sato-Okaba, K Isoda, B Heissig, T Kadoguchi, K Akita, K Kitamura, K Shimada, K Hattori, H Daida
Int J Cardiol Heart Vasc, 2020-05-10;28(0):100529.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Contribution of STAT1 to innate and adaptive immunity during type I interferon-mediated lethal virus infection
Authors: SR Jung, TM Ashhurst, PK West, B Viengkhou, NJC King, IL Campbell, MJ Hofer
PLoS Pathog., 2020-04-20;16(4):e1008525.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Csf1r or Mer inhibition delays liver regeneration via suppression of Kupffer cells
Authors: JA Santamaria, S Zeng, JB Greer, MJ Beckman, AM Seifert, NA Cohen, JQ Zhang, MH Crawley, BL Green, JK Loo, JH Maltbaek, RP DeMatteo
PLoS ONE, 2019-05-01;14(5):e0216275.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Myeloid Folliculin balances mTOR activation to maintain innate immunity homeostasis
Authors: J Li, S Wada, LK Weaver, C Biswas, EM Behrens, Z Arany
JCI Insight, 2019-03-07;5(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
ISRE-Reporter Mouse Reveals High Basal and Induced Type I IFN Responses in Inflammatory Monocytes
Authors: MB Uccellini, A García-Sas
Cell Rep, 2018-12-04;25(10):2784-2796.e3.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Production of Interleukin-20 cytokines limits bacterial clearance and lung inflammation during infection by Streptococcus pneumoniae
Authors: F Madouri, O Barada, G Kervoaze, F Trottein, M Pichavant, P Gosset
EBioMedicine, 2018-10-22;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1?
Authors: HM Hsiao, R Fernandez, S Tanaka, W Li, JH Spahn, S Chiu, M Akbarpour, D Ruiz-Perez, Q Wu, C Turam, D Scozzi, T Takahashi, HP Luehmann, V Puri, GRS Budinger, AS Krupnick, AV Misharin, KJ Lavine, Y Liu, AE Gelman, A Bharat, D Kreisel
J. Clin. Invest., 2018-05-21;128(7):2833-2847.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
SOCS3 deficiency in myeloid cells promotes retinal degeneration and angiogenesis through arginase-1 up-regulation in experimental autoimmune uveoretinitis
Authors: M Chen, J Zhao, IH Ali, S Marry, J Augustine, M Bhuckory, A Lynch, A Kissenpfen, H Xu
Am. J. Pathol., 2018-02-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration
Authors: CB Toomey, M Landowski, M Klingeborn, U Kelly, J Deans, H Dong, O Harrabi, T Van Blarco, YA Yeung, R Grishanin, JC Lin, DR Saban, C Bowes Rick
Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):662-673.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
Cancer Res, 2017-02-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion
Authors: J Liu, Y Xue, D Dong, C Xiao, C Lin, H Wang, F Song, T Fu, Z Wang, J Chen, H Pan, Y Li, D Cai, Z Li
Mucosal Immunol, 2017-01-25;0(0):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
Impact of myeloid-derived suppressor cell on Kupffer cells from mouse livers with hepatocellular carcinoma
Authors: Stéphanie Lacotte
Oncoimmunology, 2016-09-20;5(11):e1234565.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Pivotal role for skin trans-endothelial radio-resistant anti-inflammatory macrophages in tissue repair
Authors: Olga Barreiro
Elife, 2016-06-15;5(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Alternatively activated macrophages determine repair of the infarcted adult murine heart
J Clin Invest, 2016-05-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Monocyte Trafficking, Engraftment, and Delivery of Nanoparticles and an Exogenous Gene into the Acutely Inflamed Brain Tissue - Evaluations on Monocyte-Based Delivery System for the Central Nervous System
PLoS ONE, 2016-04-26;11(4):e0154022.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Donor colonic CD103+ dendritic cells determine the severity of acute graft-versus-host disease.
Authors: Koyama M, Cheong M, Markey K, Gartlan K, Kuns R, Locke K, Lineburg K, Teal B, Leveque-El Mouttie L, Bunting M, Vuckovic S, Zhang P, Teng M, Varelias A, Tey S, Wockner L, Engwerda C, Smyth M, Belz G, McColl S, MacDonald K, Hill G
J Exp Med, 2015-07-13;212(8):1303-21.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling.
Authors: Patel, Jyoti, McNeill, Eileen, Douglas, Gillian, Hale, Ashley B, de Bono, Joseph, Lee, Regent, Iqbal, Asif J, Regan-Komito, Daniel, Stylianou, Elena, Greaves, David R, Channon, Keith M
Nat Commun, 2015-03-18;6(0):6614.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
SHP-1-dependent macrophage differentiation exacerbates virus-induced myositis.
Authors: Watson N, Schneider K, Massa P
J Immunol, 2015-02-13;194(6):2796-809.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Vitamin D deficiency decreases survival of bacterial meningoencephalitis in mice.
Authors: Djukic M, Sostmann N, Bertsch T, Mecke M, Nessler S, Manig A, Hanisch U, Triebel J, Bollheimer L, Sieber C, Nau R
J Neuroinflammation, 2015-01-07;12(0):208.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.
Authors: Habbeddine M, Verbeke P, Karaz S, Bobe P, Kanellopoulos-Langevin C
PLoS ONE, 2014-09-26;9(9):e107267.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Preemptive donor apoptotic cell infusions induce IFN-gamma-producing myeloid-derived suppressor cells for cardiac allograft protection.
Authors: Bryant J, Lerret N, Wang J, Kang H, Tasch J, Zhang Z, Luo X
J Immunol, 2014-05-07;192(12):6092-101.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Collective nitric oxide production provides tissue-wide immunity during Leishmania infection.
Authors: Olekhnovitch R, Ryffel B, Muller A, Bousso P
J Clin Invest, 2014-03-10;124(4):1711-22.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Intraperitoneal prophylaxis with CpG oligodeoxynucleotides protects neutropenic mice against intracerebral Escherichia coli K1 infection.
Authors: Ribes S, Meister T, Ott M, Redlich S, Janova H, Hanisch U, Nessler S, Nau R
J Neuroinflammation, 2014-01-23;11(0):14.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The monocyte to macrophage transition in the murine sterile wound.
Authors: Crane, Meredith, Daley, Jean M, van Houtte, Olivier, Brancato, Samielle, Henry, William, Albina, Jorge E
PLoS ONE, 2014-01-22;9(1):e86660.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Calcitonin gene-related peptide regulates type IV hypersensitivity through dendritic cell functions.
Authors: Mikami, Norihisa, Sueda, Kaori, Ogitani, Yusuke, Otani, Ippei, Takatsuji, Miku, Wada, Yasuko, Watanabe, Keiko, Yoshikawa, Rintaro, Nishioka, Satoshi, Hashimoto, Nagisa, Miyagi, Yayoi, Fukada, So-ichir, Yamamoto, Hiroshi, Tsujikawa, Kazutake
PLoS ONE, 2014-01-21;9(1):e86367.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Transgenic expression of GM-CSF in T cells causes disseminated histiocytosis.
Authors: van Nieuwenhuijze A, Coghill E, Gray D, Prato S, Metcalf D, Alexander W, Wicks I
Am J Pathol, 2013-10-30;184(1):184-99.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice.
Authors: Domingos-Pereira S, Decrausaz L, Derre L, Bobst M, Romero P, Schiller J, Jichlinski P, Nardelli-Haefliger D
Mucosal Immunol, 2012-09-12;6(2):393-404.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
HMGB1 promotes recruitment of inflammatory cells to damaged tissues by forming a complex with CXCL12 and signaling via CXCR4.
Authors: Schiraldi M, Raucci A, Munoz LM, Livoti E, Celona B, Venereau E, Apuzzo T, De Marchis F, Pedotti M, Bachi A, Thelen M, Varani L, Mellado M, Proudfoot A, Bianchi ME, Uguccioni M
J. Exp. Med., 2012-02-27;209(3):551-63.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Calcitonin gene-related peptide is an important regulator of cutaneous immunity: effect on dendritic cell and T cell functions.
J. Immunol., 2011-05-06;186(12):6886-93.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Antibiotic-Induced Dysbiosis of Gut Microbiota Impairs Corneal Nerve Regeneration by Affecting CCR2-Negative Macrophage Distribution
Authors: Jun Liu, Mingjuan Wu, Jingxin He, Chengju Xiao, Yunxia Xue, Ting Fu et al.
The American Journal of Pathology
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Loss of Fractalkine Signaling Exacerbates Axon Transport Dysfunction in a Chronic Model of Glaucoma
Authors: Kevin T. Breen, Sarah R. Anderson, Michael R. Steele, David J. Calkins, Alejandra Bosco, Monica L. Vetter
Frontiers in Neuroscience
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LKB1 loss promotes endometrial cancer progression via CCL2-dependent macrophage recruitment.
Authors: Pena CG, Nakada Y, Saatcioglu HD et al.
J Clin Invest
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Organism-Level Analysis of Vaccination Reveals Networks of Protection across Tissues
Authors: Motohiko Kadoki, Ashwini Patil, Cornelius C. Thaiss, Donald J. Brooks, Surya Pandey, Deeksha Deep et al.
Cell
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CXCL10 chemokine regulates heterogeneity of the CD8+ T cell response and viral set point during chronic infection
Authors: Aleksandra J. Ozga, Melvyn T. Chow, Mateus E. Lopes, Rachel L. Servis, Mauro Di Pilato, Philippe Dehio et al.
Immunity
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Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution
Authors: Mingjuan Wu, Jun Liu, Fanying Li, Shuoya Huang, Jingxin He, Yunxia Xue et al.
Mucosal Immunology
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Single cell suspensions of mouse cells were isolated from the lung tissue and were blocked with Fc block for 30 minutes. Cells were then stained with the APC-conjugated anti-mouse CCR2 antibody (1: 100 in FACS buffer) for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry.
Data was pregated with FSC/SSC --> FSC-A/FSC-W
CCR2 expression on mouse CD8 T cell in vitro activated or virus-infected lung