Mouse CD11b/Integrin alpha M Biotinylated Antibody

Name: Mouse CD11b/Integrin alpha M Biotinylated Antibody
Brand: R&D Systems
SKU: BAM1124
Rating: 5 (1 reviews)

Catalog #: BAM1124
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAM1124

CD11b/Integrin alpha M in Mouse Spleno-cytes.

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All CD11b/Integrin alpha M Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD11b/Integrin alpha M Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD11b/Integrin alpha M. Cross-reaction with human CD11b/Integrin alpha M has been reported (1, 2).

Source

Monoclonal Rat IgG_2B Clone # M1/70

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Con A-activated C57BL/10 splenocytes

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Mouse splenocytes

Immunohistochemistry

8-25 µg/mL

Immunoprecipitation

Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CD11b/Integrin alpha M in Mouse Spleno-cytes. CD11b/Integrin aM was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin aM Biotinylated Monoclonal Antibody (Catalog # BAM1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

References

Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse CD11b/Integrin alpha M Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.
Authors: Schaeffer M, Han SJ, Chtanova T, van Dooren GG, Herzmark P, Chen Y, Roysam B, Striepen B, Robey EA
J. Immunol., 2009-05-15;182(10):6379-93.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Modulation of macrophage infiltration and inflammatory activity by the phosphatase SHP-1 in virus-induced demyelinating disease.
Authors: Christophi GP, Hudson CA, Panos M, Gruber RC, Massa PT
J. Virol., 2008-11-05;83(2):522-39.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr