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Mouse CD11b/Integrin alpha M MAb (Clone M1/70)

Catalog #: MLDP5
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Newer Version Available: MAB1124
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MLDP5 has been discontinued and is replaced by MAB1124.

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Mouse CD11b/Integrin alpha M MAb (Clone M1/70) Summary

Species Reactivity
Mouse
Specificity
This antibody reacts with mouse Integrin alpha M, the alpha subunit of MAC-1 integrin. MAC-1, the heterodimer of CD18/CD11b, is also referred to as integrin beta 2/ alpha -M, CR3 and M01. It is a receptor for fibrinogen, factor X, ICAM-1 and complement fragment C3bi.2, 3 MAC-1 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells.1 Cross-reaction of this antibody with human Integrin alpha M has been reported.4, 5
Source
Monoclonal Rat IgG2b Clone # M1/70
Label
Unconjugated

Applications

Recommended Concentration
Sample
Cell depletion
This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete Integrin alpha M+ cells from 1 x 109 bone marrow cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Cell Depletion View Larger

Cell Depletion View Larger

Figure 1:Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, Integrin alpha M, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems Catalog # MAB1356; dotted histogram) in bone marrow-progenitor cells after enrichment using the mouse lineage panel and bead depletion.

Cell Depletion View Larger

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD11b/Integrin alpha M

Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.

References
  1. Coligan, J.E. et al. in Current Protocols in Immunology, Isolation of Murine Macrophages (1994) eds., John Wiley & Sons, Inc., Volume 3, Supplement 11, 14.1.4.
Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

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Product Specific Notices

Dynabeads® is a registered trademark of Dynal Biotech, Lake Success, NY.

Citation for Mouse CD11b/Integrin alpha M MAb (Clone M1/70)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Negative regulation of monocyte adhesion to arterial elastic laminae by signal regulatory protein alpha and Src homology 2 domain-containing protein-tyrosine phosphatase-1.
    Authors: Liu SQ, Alkema PK, Tieche C, Tefft BJ, Liu DZ, Li YC, Sumpio BE, Caprini JA, Paniagua M
    J. Biol. Chem., 2005-09-13;280(47):39294-301.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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