Mouse CD11c APC-conjugated Antibody

Name: Mouse CD11c APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB69501A

Catalog #: FAB69501A Datasheet / COA / SDS

Discontinued Product

FAB69501A has been discontinued.

View all CD11c products.

Detection of CD11c in Mouse Splenocytes by Flow Cytometry.

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All CD11c Antibodies

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Mouse CD11c APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha X/CD11c.

Source

Monoclonal Hamster IgG Clone # N418

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse spleen dendritic cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD11c in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse MHC class II (I-A/I-E) Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB6118F) and either (A) Hamster Anti-Mouse CD11c APC-conjugated Monoclonal Antibody (Catalog # FAB69501A) or (B) Hamster IgG Allophycocyanin Control. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD11c

The Integrin alpha X subunit, also known as CD11c, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, hairy cell leukemias and some other leukocyte subsets. The 1097 aa mouse alpha X extracellular domain shares 71% and 87% amino acid (aa) identity with human and rat alpha X, respectively. One potential alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are shared with alpha M beta 2/CD11b/CD18 (complement iC3b, ICAMs, vWF and fibrinogen) while others (osteopontin, Thy-1, plasminogen, heparin) are unique. Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles.

References

Metlay, J.P. et al. (1990) J. Exp. Med. 171:1753.

Entrez Gene IDs

3687 (Human); 16411 (Mouse); 64350 (Rat)

Alternate Names

95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6