Mouse CD161/NK1.1 Antibody

Catalog #: MAB76141 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB76141-100
MAB76141-SP

Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD161/NK1.1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD161/NK1.1 in Flow Cytometry.

Source

Monoclonal Mouse IgG_2A Clone # PK136

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NK-1+ cells from mouse spleen and bone marrow
Accession # AAH61168

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry. Mouse C57BL/6 splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 Monoclonal Antibody (Catalog # MAB76141, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Histograms were gated on Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) positive cells. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Entrez Gene IDs

17059 (Mouse)

Alternate Names

CD161 antigen; CD161; CD161/NK1.1; CD161c; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; Klrb1c; Ly55c; Ly-59; Natural killer cell surface protein P1A; NKR-P1; NKR-P1AMGC138614; NKRP1ANKR