Mouse CD161/NK1.1 Biotinylated Antibody

Catalog #: FAB76141B Datasheet / COA / SDS

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FAB76141B-100

Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry.

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Mouse CD161/NK1.1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD161/NK1.1 in Flow Cytometry.

Source

Monoclonal Mouse IgG_2A Clone # PK136

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NK-1+ cells from mouse spleen and bone marrow
Accession # AAH61168

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

C57BL/6 splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry. Mouse C57BL/6 splenocytes were stained with (A) Mouse Anti-Mouse CD161/NK1.1 biotinylated Monoclonal Antibody (Catalog # FAB76141B) or (B) Mouse IgG2A Isotype Control (IC003B) followed by Allophycocyanin-conjugated Streptavidin (F0050) and Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (FAB22252P). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Preparation and Storage

Reconstitution

Reconstitution default not known

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Entrez Gene IDs

17059 (Mouse)

Alternate Names

CD161 antigen; CD161; CD161/NK1.1; CD161c; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; Klrb1c; Ly55c; Ly-59; Natural killer cell surface protein P1A; NKR-P1; NKR-P1AMGC138614; NKRP1ANKR