Mouse CD163 Antibody

Catalog #: MAB7435 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7435-100
MAB7435-SP

Detection of Mouse CD163 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD163 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD163 in direct ELISA.

Source

Monoclonal Rat IgG_2B Clone # 612722

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived recombinant mouse CD163
Val39-Thr1045
Accession # Q2VLH6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Mouse liver

Immunocytochemistry

3-25 µg/mL

Formalin fixed Neuro2A cells (Positive)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CD163 by Western Blot. Western Blot shows lysates of mouse liver. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD163 at approximately 140 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry

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Detection of CD163 in Neuro2A cells (Positive). CD163 was detected in fixed Neuro2A cells (Positive) using Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB7435) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD163

CD163, also known as M130 and p155, is a 130-160 kDa type I transmembrane protein belonging to group B of the cysteine-rich scavenger receptor family (1). Mature mouse CD163 consists of a 1007 amino acid (aa) extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (2). Alternative splicing of mouse CD163 generates an isoform with a substitution in the cytoplasmic region. Within the ECD, mouse CD163 shares 75% and 90% aa sequence identity with human and rat CD163, respectively. CD163 is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10 (3-5). A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage (6, 7) in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6 (8-10). CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines (11). It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin (12, 13). It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion (14). CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis (15, 16).

References

Kowal, K. et al. (2011) Folia Histochem. Cytobiol. 49:365.
Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
Hogger, P. et al. (1998) J. Immunol. 161:1883.
Sulahian, T.H. et al. (2000) Cytokine 12:1312.
Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
Etzerodt, A. et al. (2010) J. Leukoc. Biol. 88:1201.
Moreno, J.A. et al. (2012) Eur. Heart J. 33:252.
Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
Timmermann, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
Sulahian, T.H. et al. (2004) J. Leukoc. Biol. 76:271.
Fabriek, B.O. et al. (2009) Blood 113:887.
Kristiansen, M. et al. (2001) Nature 409:198.
Schaer, D.J. et al. (2006) Blood 107:373.
Fabriek, B.O. et al. (2007) Blood 109:5223.
Bover, L.C. et al. (2007) J. Immunol. 178:8183.
Moreno, J.A. et al. (2009) Atherosclerosis 207:103.

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Alternate Names

CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163