Mouse CD177 PE-conjugated Antibody Summary
Accession # Q8R2S8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD177 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rabbit Anti-Mouse CD177 PE-conjugated Monoclonal Antibody (Catalog # FAB8186P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC105P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD177
CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).
- Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
- Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
- Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
- Klippel, S. et al. (2002) Blood 100:2441.
- Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
- Temerinac, S. et al. (2000) Blood 95:2569.
- Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
- Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
- Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
- von Vietinghoff, S. et al. (2007) Blood 109:4487.
- Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
- van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.
Product Datasheets
Citations for Mouse CD177 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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CD4-mediated immunity shapes neutrophil-driven tuberculous pathology
Authors: Gern, BH;Klas, JM;Foster, KA;Cohen, SB;Plumlee, CR;Duffy, FJ;Neal, ML;Halima, M;Gustin, AT;Diercks, AH;Aderem, A;Gale, M;Aitchison, JD;Gerner, MY;Urdahl, KB;
bioRxiv : the preprint server for biology
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, Immunohistochemistry -
Thymic mimetic cells function beyond self-tolerance
Authors: Givony, T;Leshkowitz, D;Del Castillo, D;Nevo, S;Kadouri, N;Dassa, B;Gruper, Y;Khalaila, R;Ben-Nun, O;Gome, T;Dobe, J;Ben-Dor, S;Kedmi, M;Keren-Shaul, H;Heffner-Krausz, R;Porat, Z;Golani, O;Addadi, Y;Brenner, O;Lo, DD;Goldfarb, Y;Abramson, J;
Nature
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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