Mouse CD1d1 Antibody

Catalog #: AF4884 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4884
AF4884-SP

Detection of Mouse CD1d1 by Western Blot.

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Mouse CD1d1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD1d1 in direct ELISAs and Western blots. In direct ELISAs, approximately 70% cross-reactivity with recombinant mouse CD1d2 is observed, and approximately 15% cross-reactivity with recombinant human CD1d1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD1d1
Gln22-Gly305
Accession # NP_031665

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CD1d1 by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse CD1d1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4884) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD1d1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD1d1

CD1d is a 48 kDa transmembrane glycoprotein that belongs to the CD1 family of glycolipid antigen-presenting MHC-like molecules. In mouse, there are two closely related CD1d genes, CD1d1 and CD1d2, whereas human and rat have only one (1, 2). Mature mouse CD1d1 consists of a 284 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail (3). Within the ECD, mouse CD1d1 shares 94% aa sequence identity with mouse CD1d2, and 65% and 87% with human and rat CD1d, respectively. Complexes of CD1d1 with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatocytes (1). A cytoplasmic motif mediates CD1d1 recycling through the endosomal/lysosomal system where it is loaded with processed exogenous glycolipids by saposin lipid transfer proteins (4 - 8). CD1d1-presented glycolipids are recognized by canonical NKT cells that utilize an invariant V alpha 14-J alpha 18 chain in their T cell receptor (V alpha 24-J alpha 18 in human) (9, 10). NKT cells that express V chains other than alpha 14 can also recognize CD1d1-presented glycolipids but do not require them to be endosomally loaded (10, 11). NKT cells respond to a variety of CD1d1-presented glycolipids including alpha -galactosylceramide ( alpha -GalCer), a structural relative of microbial cell wall components, and the endogenous isoglobotrihexosylceramide (iGb3) (2, 9, 12). The interaction with glycolipid-loaded CD1d1 is critical for NKT cell development and induces their rapid secretion of both Th1 and Th2 type cytokines (10, 11, 13, 14). In humans, infection with HSV-1 suppresses NKT cell activation by blocking the intracellular cycling of CD1d in antigen presenting cells (15).

References