Mouse CD3 epsilon Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB484U Datasheet / COA / SDS

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FAB484U-100UG

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Mouse CD3 epsilon Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.¹ Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.^3,4

Source

Monoclonal Hamster IgG Clone # 145-2C11

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

H-2K^b-specific mouse cytotoxic T-lymphocyte

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

Mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD3 epsilon

CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.⁴ The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.¹145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.^{6, 7}

References

Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374.
Portoles, P. et al. (1989) J. Immunol. 142:4169.
Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703.
Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583.
Small, M. et al. (1994) J. Immunol. Meth. 167:103.
Alegre, M.L. et al. (1991) J. Immunol. 146:1184.
Hendrickson, M. et al. (1995) Transplantation 60:828.

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Alternate Names

CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.