Mouse CD30 Ligand/TNFSF8 Antibody

Catalog #: MAB732 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB732
MAB732-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD30 Ligand/TNFSF8 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD30 Ligand/TNFSF8 in ELISAs and Western blots. In sandwich immunoassays, no cross‑reactivity or interference with recombinant human (rh) CD30, recombinant mouse (rm) CD30, rhCD40 Ligand, rhFas Ligand, rhTNF-alpha, rmTNF-alpha, recombinant rat TNF-alpha, or rhTNF-beta is observed.

Source

Monoclonal Rat IgG_2A Clone # 82414

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD30 Ligand/TNFSF8
Gln68-Asp239
Accession # P32972

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CD30 Ligand/TNFSF8 (Catalog # 732-CL)

Mouse CD30 Ligand/TNFSF8 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse CD30 Ligand/TNFSF8 Biotinylated Antibody (Catalog # BAF732)

Standard: Recombinant Mouse CD30 Ligand/TNFSF8 Protein (Catalog # 732-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30 Ligand/TNFSF8

CD30 Ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells. The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. CD30 is also expressed on different non-Hodgkin's lymphomas, virus-infected T and B cells, and on normal T and B cells after activation. Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4⁺/CD8⁺ thymocytes that coexpress CD45RO and IL-4 receptor. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. CD30 can act as a co‑stimulatory molecule in thymic negative selection and may also play a critical role in the pathophysiology of Hodgkin's disease and other CD30⁺ lymphomas.

References

Brunangelo, F. et al. (1995) Blood 85:1.
Gruss, H-J. and F. Herrmann (1996) Leukemia and Lymphoma 20:397.
Chiarle, R. et al. (1999) J. Immunol. 163:194.

Entrez Gene IDs

944 (Human); 21949 (Mouse)

Alternate Names

CD153 antigen; CD153; CD30 antigen ligand; CD30 Ligand; CD30L; CD30-L; CD30LCD30 ligand; CD30LGMGC138144; TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand superfamily member 8