Mouse CD302/CLEC13A PE-conjugated Antibody

Name: Mouse CD302/CLEC13A PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB6424P

Catalog #: FAB6424P Datasheet / COA / SDS

Discontinued Product

FAB6424P has been discontinued.

View all CD302/CLEC13A products.

Detection of CD302/CLEC13A in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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All CD302/CLEC13A Antibodies

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Mouse CD302/CLEC13A PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD302/CLEC13A in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 662713

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD302/CLEC13A
Met1-Cys165
Accession # Q9DGC2

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD302/CLEC13A in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD302/CLEC13A PE-conjugated Monoclonal Antibody (Catalog # FAB6424P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD302/CLEC13A

CD302, also known as CLEC13A and DCL-1, is a 35 kDa type I transmembrane glycoprotein in the C-type lectin family. CD302 is expressed on monocytes, macrophages, dendritic cells, and granulocytes. It contains one carbohydrate binding domain in its extracellular region and can mediate antigen uptake. In human Hodgkin’s disease, a fusion protein containing portions of CD302 and the ECD of DEC-205 is expressed on Reed-Sternberg cells. Within the ECD, mouse CD302 shares 82% and 92% aa sequence identity with human and rat CD302, respectively.

Entrez Gene IDs

9936 (Human); 66205 (Mouse); 295629 (Rat)

Alternate Names

BIMLEC; CD302 antigenC-type lectin domain family 13, member A; CD302 molecule; CD302; CLEC13A; CLEC13AMGC22301; C-type lectin domain family 13 member A; DCL1; DCL-1; DCL1C-type lectin BIMLEC; KIAA0022FLJ43091; Type I transmembrane C-type lectin receptor DCL-1