Mouse CD36/SR-B3 Antibody Summary
Gly30-Lys439
Accession # Q08857
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse CD36/SR-B3 Monoclonal Antibody (Catalog # MAB25191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
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CD36/SR‑B3 in Mouse Heart. CD36/SR-B3 was detected in immersion fixed frozen sections of mouse heart using Rat Anti-Mouse CD36/SR-B3 Monoclonal Antibody (Catalog # MAB25191) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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CD36/SR‑B3 in Mouse Thymus. CD36/SR-B3 was detected in immersion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD36/SR-B3 Monoclonal Antibody (Catalog # MAB25191) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD36/SR-B3
CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 shares 94%, 92%, 84% and 84% aa sequence identity with the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).
- Febbraio, M. et al. (2001) J. Clin. Invest. 108:795.
- Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483.
- Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446.
- Endemann, G. et al. (1993) J. Biol. Chem. 268:11811.
- Malaud, E. et al. (2002) Biochem. J. 364:507.
- Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
- Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
- Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.
Product Datasheets
Citations for Mouse CD36/SR-B3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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An NKX2-1/ERK/WNT feedback loop modulates gastric identity and response to targeted therapy in lung adenocarcinoma
Authors: Rediet Zewdu, Elnaz Mirzaei Mehrabad, Kelley Ingram, Pengshu Fang, Katherine L Gillis, Soledad A Camolotto et al.
eLife
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Macrophage Transcriptional Profile Identifies Lipid Catabolic Pathways That Can Be Therapeutically Targeted after Spinal Cord Injury
Authors: Y. Zhu, K. Lyapichev, D.H. Lee, D. Motti, N.M. Ferraro, Y. Zhang et al.
The Journal of Neuroscience
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CD36 deletion reduces VLDL secretion, modulates liver prostaglandins, and exacerbates hepatic steatosis in ob/ob mice.
Authors: Nassir F, Adewole O, Brunt E, Abumrad N
J Lipid Res, 2013-08-20;54(11):2988-97.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hematopoietic cell-restricted deletion of CD36 reduces high-fat diet-induced macrophage infiltration and improves insulin signaling in adipose tissue.
Authors: Nicholls HT, Kowalski G, Kennedy DJ
Diabetes, 2011-03-04;60(4):1100-10.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Meningeal Foam Cells and Ependymal Cells in Axolotl Spinal Cord Regeneration
Authors: Enos N, Takenaka H, Scott S et al.
Front Immunol.
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