Mouse CD39/ENTPD1 Antibody

Catalog # Availability Size / Price Qty
AF4398
AF4398-SP

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Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry.
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Citations (5)
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Mouse CD39/ENTPD1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human CD39 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1
Thr38-Ile478
Accession # Q921Q6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD39/ENTPD1 (Catalog # 4398-EN)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse CD39/ENTPD1 (Catalog # 4398-EN), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD39/ENTPD1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse CD39/ENTPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4398, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Immunohistochemistry CD39/ENTPD1 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

CD39/ENTPD1 in Mouse Liver. CD39/ENTPD1 was detected in perfusion fixed frozen sections of mouse liver using Mouse CD39/ENTPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4398) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. Recombinant mouse NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (1), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (2). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (3). NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation (4). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (5).

References
  1. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
  2. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
  3. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
  4. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
  5. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
Entrez Gene IDs
953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)
Alternate Names
ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1

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Citations for Mouse CD39/ENTPD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss
    Authors: Yu-Ru V. Shih, Mengqian Liu, Seong Keun Kwon, Masayuki Iida, Ya Gong, Nivedita Sangaj et al.
    Science Advances
  2. SARS-CoV-2 Spike protein alters microglial purinergic signaling
    Authors: Vinícius Santos Alves, Stephanie Alexia Cristina Silva Santos, Raíssa Leite-Aguiar, Elaine Paiva-Pereira, Renata Rodrigues dos Dos Reis, Mariana L. Calazans et al.
    Frontiers in Immunology
  3. Negative feedback control of neuronal activity by microglia
    Authors: A Badimon, HJ Strasburge, P Ayata, X Chen, A Nair, A Ikegami, P Hwang, AT Chan, SM Graves, JO Uweru, C Ledderose, MG Kutlu, MA Wheeler, A Kahan, M Ishikawa, YC Wang, YE Loh, JX Jiang, DJ Surmeier, SC Robson, WG Junger, R Sebra, ES Calipari, PJ Kenny, UB Eyo, M Colonna, FJ Quintana, H Wake, V Gradinaru, A Schaefer
    Nature, 2020-09-30;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. P2X7 receptor activation increases caveolin-1 expression and macrophage lipid raft formation boosting CD39 activity
    Authors: LEB Savio, P de Andrade, SACS Santos, JC de Sousa, SDS Oliveira, RD Minshall, E Kurtenbach, Y Wu, MS Longhi, SC Robson, R Coutinho-S
    J. Cell. Sci., 2020-03-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Complete deletion of Cd39 is atheroprotective in apolipoprotein E-deficient mice
    Authors: M De Giorgi, K Enjyoji, G Jiang, E Csizmadia, S Mitsuhashi, RJ Gumina, RT Smolenski, SC Robson
    J. Lipid Res., 2017-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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