Home / CD39/ENTPD1 / Mouse CD39/ENTPD1 Antibody

Mouse CD39/ENTPD1 Antibody

Catalog #: AF4398
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4398
AF4398-SP
Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD39/ENTPD1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human CD39 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1
Thr38-Ile478
Accession # Q921Q6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD39/ENTPD1 (Catalog # 4398-EN)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse CD39/ENTPD1 (Catalog # 4398-EN), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD39/ENTPD1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse CD39/ENTPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4398, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Immunohistochemistry CD39/ENTPD1 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

CD39/ENTPD1 in Mouse Liver. CD39/ENTPD1 was detected in perfusion fixed frozen sections of mouse liver using Mouse CD39/ENTPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4398) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. Recombinant mouse NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (1), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (2). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (3). NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation (4). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (5).

References
  1. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
  2. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
  3. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
  4. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
  5. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
Entrez Gene IDs
953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)
Alternate Names
ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1

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Citations for Mouse CD39/ENTPD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss
    Authors: Yu-Ru V. Shih, Mengqian Liu, Seong Keun Kwon, Masayuki Iida, Ya Gong, Nivedita Sangaj et al.
    Science Advances
  2. SARS-CoV-2 Spike protein alters microglial purinergic signaling
    Authors: Vinícius Santos Alves, Stephanie Alexia Cristina Silva Santos, Raíssa Leite-Aguiar, Elaine Paiva-Pereira, Renata Rodrigues dos Dos Reis, Mariana L. Calazans et al.
    Frontiers in Immunology
  3. Negative feedback control of neuronal activity by microglia
    Authors: A Badimon, HJ Strasburge, P Ayata, X Chen, A Nair, A Ikegami, P Hwang, AT Chan, SM Graves, JO Uweru, C Ledderose, MG Kutlu, MA Wheeler, A Kahan, M Ishikawa, YC Wang, YE Loh, JX Jiang, DJ Surmeier, SC Robson, WG Junger, R Sebra, ES Calipari, PJ Kenny, UB Eyo, M Colonna, FJ Quintana, H Wake, V Gradinaru, A Schaefer
    Nature, 2020-09-30;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. P2X7 receptor activation increases caveolin-1 expression and macrophage lipid raft formation boosting CD39 activity
    Authors: LEB Savio, P de Andrade, SACS Santos, JC de Sousa, SDS Oliveira, RD Minshall, E Kurtenbach, Y Wu, MS Longhi, SC Robson, R Coutinho-S
    J. Cell. Sci., 2020-03-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Complete deletion of Cd39 is atheroprotective in apolipoprotein E-deficient mice
    Authors: M De Giorgi, K Enjyoji, G Jiang, E Csizmadia, S Mitsuhashi, RJ Gumina, RT Smolenski, SC Robson
    J. Lipid Res., 2017-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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