Mouse CD39L2/ENTPD6 Antibody

Catalog #: MAB6288 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6288-100
MAB6288-SP

Detection of CD39L2/ENTPD6 in CHO-S transfected cells.

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Mouse CD39L2/ENTPD6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD39L2/ENTPD6 in direct ELISA.

Source

Monoclonal Rat IgG_2B Clone # 774308

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived mouse CD39L2/ENTPD6
Lys33-Leu455
Accession # 742115

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Mouse Testis

Immunocytochemistry

8-25 µg/mL

Immersion fixed CHO-S transfected (positive) and CHO-S wild type (negative) cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of CD39L2/ENTPD6 in CHO-S transfected cells. CD39L2/ENTPD6 was detected in immersion fixed CHO-S transfected (positive) and absent in CHO-S wild type (negative) cells using Rat Anti-Mouse CD39L2/ENTPD6 Monoclonal Antibody (Catalog # MAB6288) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Detection of CD39L2/ENTPD6 in Mouse Testis. CD39L2/ENTPD6 was detected in immersion fixed paraffin-embedded sections of Mouse Testis using Rat Anti-Mouse CD39L2/ENTPD6 Monoclonal Antibody (Catalog # MAB6288) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in late spermatids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L2/ENTPD6

CD39L2, also known as ectonucleoside triphosphate diphosphohydrolase-6 (ENTPD-6) and NTPDase-6, is a nucleoside phosphohydrolase of the CD39 family of enzymes that is present on the surface of cells (1). This Type II membrane protein also exists as a soluble, secreted protein. CD39L2 displays a preference for the nucleoside-5’-diphosphates GDP and IDP over CDP and UDP (2). Nucleoside-5’-triphosphates are also hydrolyzed, but at much lower rates. CD39L2 is expressed at its highest levels in cardiac muscle and in heart capillary endothelial cells (3). CD39L2 may play a functional role in the regulation and recruitment of platelet activation in the heart. The cytoplasmic and transmembrane domains of rmCD39L2 were replaced with a signal sequence, resulting in the secretion of the recombinant protein.

References

Chadwick, B.P. and A.M. Frischauf (1998) Genomics. 50:357.
Hicks-Berger, C.A. et al. (2000) J.Biol. Chem. 275:34041.
Yeung, G. et al. (2000) Biochemistry. 39:12916.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 6

Entrez Gene IDs

955 (Human); 12497 (Mouse); 85260 (Rat)

Alternate Names

CD39 antigen-like 2; CD39L2; CD39L2NTPDase-6; CD39-like 2; dJ738P15.3; DKFZp781G2277; DKFZp781K21102; EC 3.6.1.6; ectonucleoside triphosphate diphosphohydrolase 6 (putative function); ectonucleoside triphosphate diphosphohydrolase 6 (putative); ectonucleoside triphosphate diphosphohydrolase 6; ENTPD6; FLJ36711; IL-6SAG; IL6ST2; interleukin 6 signal transducer-2; NTPD3; NTPDase 6