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Mouse CD72 Biotinylated Antibody

Catalog #: BAF1279 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF1279
CD72 in Mouse Splenocytes.
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Mouse CD72 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD72 in Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human CD72 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD72
Arg117-Asn354
Accession # P21855
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD72
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD72 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD72 in Mouse Splenocytes. CD72 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD72 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD72

CD72, also named B-cell differentiation antigen Lyb-2, is a Type II transmembrane protein with a C-type lectin domain. It associates with CD5 and is a functional receptor for Semaphorin 4D in the immune system.

Entrez Gene IDs
971 (Human); 12517 (Mouse)
Alternate Names
CD72 antigenB-cell differentiation antigen CD72; CD72 molecule; CD72; CD72b; Ly-19.2; Ly-32.2; Lyb-2; LYB2lyb-2

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