Mouse CD79A Antibody

Catalog #: AF6968 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6968
AF6968-SP

Detection of Mouse CD79A by Western Blot.

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Mouse CD79A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD79A in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human CD79A is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD79A
Leu29-Arg137
Accession # P11911

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CD79A by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD79A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD79A at approximately 32-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD79A

CD79A (also known as Mb-1, Ig alpha and B cell antigen receptor complex-associated protein alpha -chain) is a 30-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79B. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79A:B initiates signaling. CD79A is essential for the differentiation of pre-B cells, and the pre-BCR regulates the surface expression of IL-7R. Mature mouse CD79A is a 192 amino acid (aa) type I transmembrane glycoprotein (aa 29-220). It contains an extracellular region (aa 29-137) with one C2-type Ig-like domain (aa 29-117), and an ITAM-containing cytoplasmic domain (aa 171-199). Mouse CD79A and CD79B share only 22% aa identity. Over aa 29-137, mouse CD79A shares 57% and 80% aa identity with human and rat CD79A, respectively.

Entrez Gene IDs

973 (Human); 12518 (Mouse); 681236 (Rat); 722190 (Rhesus Macaque)

Alternate Names

CD79A antigen (immunoglobulin-associated alpha); CD79a antigen; CD79a molecule, immunoglobulin-associated alpha; CD79A; IGAB-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; MB1; MB-1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein