Mouse CD81 Antibody

Catalog #: MAB4865 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4865
MAB4865-SP

Detection of CD81 in Mouse Splenocytes by Flow Cytometry.

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Mouse CD81 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD81. Stains mouse CD81 transfectants but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2A Clone # 431301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with mouse CD81
Met1-Tyr236
Accession # NP_598416

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD81 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD81 Monoclonal Antibody (Catalog # MAB4865, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD81

CD81, also known as TAPA-1 and Tspan28, is a widely expressed protein in the Tetraspanin family. CD81 is a multifunctional protein that interacts with a variety of other molecules, including Tetraspanins, and is important for organization of the plasma membrane into microdomains. CD81 facilitates B cell and T cell activation and is an integrin-binding adhesion molecule. CD81 expression on lymphocytes is altered during infection by hepatitis C virus or HIV-1 and contributes to the pathogenicity of those viruses. Mouse CD81 shares 92%‑94% aa sequence identity with human and rat CD81.

Entrez Gene IDs

975 (Human); 12520 (Mouse); 25621 (Rat)

Alternate Names

CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7