Mouse CD90/Thy1 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB7335G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB7335G-100UG

Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry.

1 Image

All CD90/Thy1 Antibodies

Product Details

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD90/Thy1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD90/Thy1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD90/Thy1 is observed.

Source

Monoclonal Rat IgG_2A Clone # 778053

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD90/Thy1
Gln20-Lys130
Accession # P01831

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD90/Thy1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7335G) or (B) Rat IgG_2AAlexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD90/Thy1

CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Alternate Names

CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1