Mouse CD97 v2 Antibody

Catalog #: MAB3734
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3734
MAB3734-SP

Detection of CD97 in Mouse Splenocytes by Flow Cytometry.

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All CD97 Antibodies

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Mouse CD97 v2 Antibody Summary

Species Reactivity

Mouse

Specificity

In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed.

Source

Monoclonal Rat IgG₁ Clone # 587702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD97v2
Gln24-His384
Accession # AAH06676

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Mouse CD97 Variant 2 (Catalog # 5494-CD) to immobilized Recombinant Mouse CD55/DAF (Catalog # 5490-CD) coated at 0.5 µg/mL (100 µL/well). At 5 µg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD97 in Mouse Splenocytes by Flow Cytometry. Mouse CD11c+ splenocytes were stained with Rat Anti-Mouse CD97 Monoclonal Antibody (Catalog # MAB3734, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD97

CD97 is an approximately 100 kDa N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. It contains a 510 amino acid (aa) N-terminal extracellular region with four EGF-like domains and a juxtamembrane GPS motif which enables proteolytic shedding of the N-terminal extracellular domain. The alternately spliced variant 2 of CD97 exhibits a 94 aa deletion that results in loss of the third EGF-like repeat. CD97 is expressed on monocytes, macrophages, T cells, B cell subsets, dendritic cells, hematopoietic progenitor cells, and smooth muscle cells. It binds CD55/DAF, chondroitin sulfate, and Integrin although interaction with these molecules can vary between splice forms of CD97. CD97 participates in neutrophil migration, host defense, and angiogenesis. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively.

Entrez Gene IDs

976 (Human); 26364 (Mouse)

Alternate Names

CD97; TM7LN1

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Citation for Mouse CD97 v2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1