Mouse CDCP1 APC-conjugated Antibody

Name: Mouse CDCP1 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB45151A

Catalog #: FAB45151A Datasheet / COA / SDS

Discontinued Product

FAB45151A has been discontinued.

View all CDCP1 products.

Detection of CDCP1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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Mouse CDCP1 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CDCP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CDCP1 is observed.

Source

Monoclonal Rat IgG₁ Clone # 469114

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CDCP1
Arg25-Ala667
Accession # Q5U462

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CDCP1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CDCP1 APC-conjugated Monoclonal Antibody (Catalog # FAB45151A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CDCP1

CUB domain containing protein 1 (CDCP1), also known as SIMA135, is a 140 kDa type I transmembrane glycoprotein with three CUB protein‑protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Long Name

CUB Domain Containing Protein 1

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Alternate Names

CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases