Mouse Chitotriosidase/CHIT1 Biotinylated Antibody

Catalog #: BAF5325 Datasheet / COA / SDS

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BAF5325

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Mouse Chitotriosidase/CHIT1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Chitotriosidase/CHIT1 in Western blots. In Western blots, approximately 30% cross‑reactivity with recombinant human CHIT1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Chitotriosidase/CHIT1
Ala22-Ser464
Accession # Q9D7Q1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Chitotriosidase/CHIT1 (Catalog # 5325-GH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitotriosidase/CHIT1

Chitotriosidase, encoded by the mouse CHIT1 gene, is a typical member of the chitinase family (1). It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract (2). CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form (2‑4). Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease (5). The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme (ACE) (5). Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (6).

References

Zheng, T. et al. (2005) Gene 357:37.
Boot, R.G. et al. (2006) J. Histochem. Cytochem. 53:1283.
Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Entrez Gene IDs

1118 (Human)

Alternate Names

CHI3; CHIT1; CHITCHI3; chitinase 1 (chitotriosidase); chitinase-1; Chitotriosidase; chitotriosidase-1; EC 3.2.1.14; FLJ00314; MGC125322; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase