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Mouse Chordin Antibody

Catalog #: AF758
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF758
AF758-SP
Chordin in Mouse Liver.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Chordin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Chordin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Chordin
Thr27-Ser948
Accession # Q9Z0E2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Chordin (Catalog # 758-CN)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 3-12 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP) to immobilized Recombinant Mouse Chordin (Catalog # 758-CN) coated at 5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Chordin antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

Chordin in Mouse Liver. Chordin was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse Chordin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF758) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin

Chordin is a secreted glycoprotein that regulates dorsoventral patterning during gastrulation. Chordin functions as a bone morphogenetic protein (BMP) antagonist that blocks their ventralizing activity by binding to the BMPs and inhibiting their interaction with their receptors. Mouse Chordin cDNA encodes a 948 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide. Chordin contains four internal cysteine-rich repeats (CRs) that are conserved in the spacing of their ten cysteine residues. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding. These conserved CRs are present in an expanding family of secreted molecules that antagonize BMP signaling. Xolloid (an extracellular zinc metalloproteinase) can cleave chordin at two specific sites resulting in chordin fragments with lower BMP-affinity. Cleavage of the chordin/BMP complex can reverse the BMP antagonist activity of chordin. Mouse chordin is expressed at high levels in 7 day postcoitum mouse embryos. Chordin expression is also detected in multiple fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles for chordin in organogenesis and homeostasis.

References
  1. De Robertis, E.M. and Y. Sasai (1996) Nature 380:37.
  2. Larrain, J. et al. (2000) Development 127:821.
  3. Coffinier, C. et al. (2001) Mech. Dev. 100:119.
Entrez Gene IDs
8646 (Human); 12667 (Mouse)
Alternate Names
Chordin; CHRD; MGC133038; Sog

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Citations for Mouse Chordin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. A Multiplexed Barcodelet Single-Cell RNA-Seq Approach Elucidates Combinatorial Signaling Pathways that Drive ESC Differentiation
    Authors: GHT Yeo, L Lin, CY Qi, M Cha, DK Gifford, RI Sherwood
    Cell Stem Cell, 2020-05-26;26(6):938-950.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Oct4 is required ~E7.5 for proliferation in the primitive streak.
    Authors: DeVeale B, Brokhman I, Mohseni P, Babak T, Yoon C, Lin A, Onishi K, Tomilin A, Pevny L, Zandstra P, Nagy A, van der Kooy D
    PLoS Genet, 2013-11-14;9(11):e1003957.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
    Authors: Zakin L, Chang EY, Plouhinec JL, De Robertis EM
    Dev. Biol., 2010-08-31;347(1):204-15.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  4. Chordin-induced lineage plasticity of adult SVZ neuroblasts after demyelination.
    Authors: Jablonska B, Aguirre A, Raymond M, Szabo G, Kitabatake Y, Sailor KA, Ming GL, Song H, Gallo V
    Nat. Neurosci., 2010-04-25;13(5):541-50.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
    Authors: Reversade B, De Robertis EM
    Cell, 2005-12-16;123(6):1147-60.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot

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