Mouse CL-P1/COLEC12 APC-conjugated Antibody
Mouse CL-P1/COLEC12 APC-conjugated Antibody Summary
Ala101-Leu742
Accession # Q8K4Q8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of CL‑P1/COLEC12 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CL-P1/COLEC12 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3130A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CL-P1/COLEC12
Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Collectin Placenta 1 (CL-P1), also known as Collectin sub-family member 12 and Scavenger Receptor with C-type Lectin type I (SRCL), is a 140 kDa member of the collectin family of glycoproteins. With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL‑L1 (Collectin Liver-1) is the only known cytoplasmic collectin (1). Mouse CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that includes an N-terminal 39 aa cytoplasmic domain, an 18 aa transmembrane segment, and a 685 aa C-terminal extracellular domain. The short cytoplasmic domain contains an internalization motif (Y-K-R-F), while the ECD is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure, and a C‑type lectin/Carbohydrate Recognition Domain (CRD) (5, 6). Unlike human CL-P1, no splice variants of mouse CL-P1 have been described (5, 7). Trimerization of CL‑P1 is mediated by its collagen-like and coiled-coil helical domains (1, 6). Within the ECD, mouse CL-P1 shares 88%, 89%, 92%, and 98% aa sequence identity with bovine, canine, human, and rat CL-P1, respectively. The CRD shares 23‑27% aa sequence identity with the CRD of collectins CL-L1, collectin sub-family member 11, MBL, SP-A1, and SP-D. Notably, this CRD recognizes galactose and fucose within the context of asialo-orosomucoids associated with the Lewisx epitope (8, 9). CL‑P1 is expressed in vascular endothelial cells and may play a role in bacterial recognition or as a scavenger receptor for desialylated glycoproteins (6, 8).
- van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
- Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
- Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
- Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
- Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
- Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
- Nakamura, K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
- Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
- Yoshida, T. et al. (2003) J. Biochem. 133:271.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse CL-P1/COLEC12 APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Mouse CL-P1/COLEC12 APC-conjugated Antibody and earn rewards!
Have you used Mouse CL-P1/COLEC12 APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
