Mouse COCO Antibody

Catalog #: MAB3356 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3356
MAB3356-SP

All COCO Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse COCO Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse COCO in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) COCO, rhDAN, recombinant mouse (rm) Gremlin, rmCerberus, and recombinant chicken Caronte is observed.

Source

Monoclonal Rat IgG_2A Clone # 340531

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse COCO
Arg24-Leu185
Accession # Q76LW6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse COCO (Catalog # 3356-CC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COCO

COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine knot domain that is homologous to that found in other TGF-beta superfamily ligands such as BMPs that play important roles in tissue morphogenesis and developmental processes (1‑6). The mouse COCO cDNA encodes a 185 amino acid (aa) precursor with a 23 aa signal sequence (7, 8). COCO has eight Cys residues in the cysteine knot which places it in the CAN (or eight-membered ring) subfamily of BMP antagonists along with the other DAN family proteins (1). Mature mouse COCO shares 62% and 27% aa sequence identity with human and Xenopus COCO, respectively. It shares 22%‑27% aa sequence identity with mouse DAN, Gremlin, PRDC, and Cerberus. In Xenopus embryos, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO is required for Xenopus left-right axis formation (9). It functions predominantly on the right side of the embryo, although it is equally expressed on both left and right sides (9). COCO binds and inhibits activin, BMP-4, GDF-3/derrière, Wnt8, and Xnr1 (7, 9). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8).

References

Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423.
Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885.
Baron, M.H. (2005) Exp. Hematol. 33:1015.
Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285.
Bell, E. et al. (2003) Development 130:1381.
Pearce, J.J. et al. (1999) Dev. Biol. 209:98.
Vonica, A. and A.H. Brivanlou (2007) Dev. Biol. 303:281.

Entrez Gene IDs

199699 (Human); 23863 (Mouse); 685719 (Rat)

Alternate Names

CER2CRL2; Cerberus 2; Cerberus like-2; cerberus-like 2; Cerberus-like protein 2; CERL2; cerl-2; CKTSF1B3; CKTSF1B3MGC126849; COCO; Cysteine knot superfamily 1, BMP antagonist 3; DAN domain family member 5; DAN domain family, member 5; DAND5; Dante; DTE; FLJ38607; GREM3; GREM3cerberus 2; gremlin-3; SP1