Mouse COCO Biotinylated Antibody

Catalog #: BAF3356 Datasheet / COA / SDS

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BAF3356

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Mouse COCO Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse COCO in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human COCO is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli- and mouse myeloma cell line NS0-derived recombinant mouse COCO
Accession # NP_95679

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse COCO (Catalog # 3356-CC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COCO

COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine knot domain that is homologous to that found in other TGF-beta superfamily ligands such as BMPs that play important roles in tissue morphogenesis and developmental processes (1‑6). The mouse COCO cDNA encodes a 185 amino acid (aa) precursor with a 23 aa signal sequence (7, 8). COCO has eight Cys residues in the cysteine knot which places it in the CAN (or eight-membered ring) subfamily of BMP antagonists along with the other DAN family proteins (1). Mature mouse COCO shares 62% and 27% aa sequence identity with human and Xenopus COCO, respectively. It shares 22%‑27% aa sequence identity with mouse DAN, Gremlin, PRDC, and Cerberus. In Xenopus embryos, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO is required for Xenopus left-right axis formation (9). It functions predominantly on the right side of the embryo, although it is equally expressed on both left and right sides (9). COCO binds and inhibits activin, BMP-4, GDF-3/derrière, Wnt8, and Xnr1 (7, 9). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8).

References

Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423.
Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885.
Baron, M.H. (2005) Exp. Hematol. 33:1015.
Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285.
Bell, E. et al. (2003) Development 130:1381.
Pearce, J.J. et al. (1999) Dev. Biol. 209:98.
Vonica, A. and A.H. Brivanlou (2007) Dev. Biol. 303:281.

Entrez Gene IDs

199699 (Human); 23863 (Mouse); 685719 (Rat)

Alternate Names

CER2CRL2; Cerberus 2; Cerberus like-2; cerberus-like 2; Cerberus-like protein 2; CERL2; cerl-2; CKTSF1B3; CKTSF1B3MGC126849; COCO; Cysteine knot superfamily 1, BMP antagonist 3; DAN domain family member 5; DAN domain family, member 5; DAND5; Dante; DTE; FLJ38607; GREM3; GREM3cerberus 2; gremlin-3; SP1