Mouse Contactin-3 Antibody

Catalog #: MAB6644 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6644
MAB6644-SP

Detection of Mouse Contactin‑3 by Western Blot.

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All Contactin-3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Contactin-3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Contactin‑3 in direct ELISAS. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) Contactin-3 and no cross‑reactivity with recombinant mouse (rm) Contactin-1, -4, -6, rhContactin-2 or -5 is observed.

Source

Monoclonal Rat IgG_2A Clone # 524518

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Contactin‑3
Glu20-Thr1003
Accession # Q07409

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Contactin‑3 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Contactin-3 Monoclonal Antibody (Catalog # MAB6644) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Contactin-3 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.

Flow Cytometry

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Detection of CNTN-3 in bEnd.3 mouse endothelioma cell line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Contactin-3 Monoclonal Antibody (Catalog # MAB6644, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-3

Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons, and olfactory granule cells. It likely mediates cell-cell interaction and may promote neurite outgrowth. The mouse Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region, and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG-like domains (aa 32-593), followed by four Fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, mouse Contactin-3 shares 92% and 96% aa identity with human and rat Contactin-3, respectively.

Entrez Gene IDs

5067 (Human); 18488 (Mouse); 54279 (Rat)

Alternate Names

BIG-1; CNTN3; contactin 3 (plasmacytoma associated); Contactin3; Contactin-3; PANG; PCS