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Mouse CRISP-4 Antibody

Catalog #: AF5017
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5017
AF5017-SP
Detection of Mouse CRISP‑4 by Western Blot.
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Citations (1)
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Mouse CRISP-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CRISP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse CRISP-1, -2, and -3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CRISP-4
Leu23-Lys250
Accession # NP_084309
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CRISP‑4 antibody by Western Blot. View Larger

Detection of Mouse CRISP‑4 by Western Blot. Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CRISP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5017) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CRISP-4 at approximately 26-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-4

CRISP‑4 (cysteine‑rich secretory protein 4) is a secreted, 31 kDa member of the CRISP family of proteins. In mouse, its expression is limited to the epididymis, seminal vesicle and vas deferens and it appears to bind to differentiating sperm. Mature mouse CRISP‑4 is 230 amino acids (aa) in length. It contains an SCP (sperm coating protein) region (aa 42‑178) followed by a C‑terminal CRISP domain (aa 196‑250). There appears to be an alternate start site four amino acids upstream of the standard start site. Over aa 22‑550, mouse CRISP‑4 shares 90% aa identity with rat CRISP‑4 and 64% aa identity with human CRISP‑1, the human ortholog to mouse CRISP‑4.

Long Name
Cysteine-rich Secretory Protein 4
Entrez Gene IDs
78081 (Mouse); 654517 (Rat)
Alternate Names
CRISP4; CRISP-4

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Citation for Mouse CRISP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Impaired male fertility and abnormal epididymal epithelium differentiation in mice lacking CRISP1 and CRISP4
    Authors: G Carvajal, NG Brukman, M Weigel Muñ, MA Battistone, VA Guazzone, M Ikawa, M Haruhiko, L Lustig, S Breton, PS Cuasnicu
    Sci Rep, 2018-12-03;8(1):17531.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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