Mouse Crossveinless-2/CV-2 Biotinylated Antibody

Catalog #: BAF2299 Datasheet / COA / SDS

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BAF2299

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Mouse Crossveinless-2/CV-2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Crossveinless‑2/CV‑2 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human CV-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Crossveinless‑2/CV‑2
Val34-Arg685
Accession # AAH66153

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Crossveinless‑2/CV‑2 (Catalog # 2299-CV)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Crossveinless-2/CV-2

Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted chordin-like protein that modulates the BMP signaling pathway (1‑3). Mouse CV-2 is synthesized as a 685 amino acid (aa) residue precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 2, 4). Secreted CV-2 is reported to be proteolytically cleaved to generate two fragments that are disulfide-linked (1, 2). The GDPH sequence is conserved in CV-2 from other species. It is also found in multiple proteins that undergo a similar type of cleavage (5). Mouse CV-2 message is detected in many tissues, with the highest expression detected in the heart, lungs, and skin (2). It is also expressed in flk-1⁺ endothelial cell precursors and in primary chondrocytes (2). During embryonic development, CV-2 is expressed in the dorsal midline, regions of the telencephalon, migrating cells of the branchial neural crest and endothelial cells in the yolk sac (2). Mouse CV-2 shares 92% and 34% aa sequence identity with the human and Drosophila homologs, respectively (1, 4). Results from biochemical experiments using recombinant CV-2 show that CV-2 directly interacts with BMP-2, -4, and -6 to antagonize BMP signaling, which can regulate a wide range of differentiation processes (1, 2). In contrast, genetic data from Drosophila suggest that CV-2 potentiates BMP-signaling (6). It is possible that like TSG, CV-2 can positively and negatively modulate BMP signal transduction depending on the cell context (7).

References

Binnerts, M.E. et al. (2004) Biochem Biophys Res Commun. 315:272.
Moser, M. et al. (2003) Mol Cell Biol. 23:5664.
Garcia-Abreu, J. et al. (2002) Gene 287:39.
Coffinier, C. et al. (2002) Mech Dev. 119:S179.
Lidell, M.E. et al. (2003) J. Biol. Chem. 278:13944.
Conley, C.A. et al. (2000) Development 127:3947.
Kamimura, M. et al. (2004) Developmental Dynamics 230:434.

Long Name

BMP-binding Endothelial Regulator Protein

Entrez Gene IDs

168667 (Human); 73230 (Mouse)

Alternate Names

BMP binding endothelial regulator; BMP-binding endothelial regulator precursor protein; BMP-binding endothelial regulator protein; BMPER; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; CRIM3; crossveinless 2; Crossveinless-2; CV2; CV-2; hCV2; KIAA1965; Protein crossveinless-2