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Mouse Cryptic Antibody

Catalog #: AF1840 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1840
AF1840-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cryptic Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cryptic in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human Cryptic is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cryptic
Tyr36-Gly177
Accession # P97766
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Cryptic
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cryptic

Cryptic, also known as CFC-1, is a member of the EGF-CFC family. It exists both as a secreted and as a GPI-anchored protein. Cryptic functions as a cofactor that modulates the activities of some members of the TGF-beta family.

Entrez Gene IDs
55997 (Human); 12627 (Mouse)
Alternate Names
CFC1; CFC-1; CFC1B; cripto, FRL-1, cryptic family 1; Cryptic family protein 1; cryptic protein; Cryptic; CRYPTICFLJ77897; HTX2MGC133213

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