Mouse CTLA-4 APC-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CTLA-4

CTLA-4 (also known as CD152) and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CTLA-4 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CTLA-4 and CD28 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CTLA-4 is expressed on the cell surface as either a disulfide-linked homodimer or a 24-26 kDa monomer. CTLA-4 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CTLA-4 transcripts have since been found in both Th1 and Th2, and CD4⁺ and CD8⁺ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4⁺ T cells and 50% of CD8⁺ T cells, and is down regulated upon T cell activation, CTLA-4 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with 20-100-fold higher affinity than CD28. The physiological role of CTLA-4 in T cell costimulation is contextually defined. Full-length CTLA-4, when induced and activated, sends an inhibitory signal to the expressing T cell. It also promotes the development of T_c17 cells. Tregs constitutively express CTLA-4, a phenotype that is essential to their function. Over amino acids (aa) 36-161, mouse CTLA-4 shares 94% and 67% aa sequence identity with rat and human CTLA-4, respectively.

Product Datasheets

Related Research Areas

• B Cell Activation
• Co-stimulatory and Co-inhibitory Molecules
• Dendritic Cell Co-stimulatory Molecules
• ITIM/ITAM Immunoreceptors and Related Molecules
• Regulatory T Cells (Tregs)
• Search Immune Checkpoint Targets