Mouse CTLA-4 Fluorescein-conjugated Antibody
Mouse CTLA-4 Fluorescein-conjugated Antibody Summary
Ala36-Phe161
Accession # XP_001479180
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of CTLA-4 antibody in Mouse Splenocytes antibody by Flow Cytometry. Detection of CTLA-4 antibody in Mouse Splenocytes antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/CTLA4_FAB434F_Flow_Cytometry_15705.jpg)
Detection of CTLA‑4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with Concanavalin A for 48 hours were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CTLA-4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB434F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CTLA-4
CTLA-4 and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CTLA-4 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CTLA-4 and CD28 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CTLA-4 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2. CTLA-4 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CTLA-4 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CTLA-4 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with 20-100-fold higher affinity than CD28. The physiological role of CTLA-4 in T cell costimulation is currently being studied. Recombinant human or mouse CTLA-4/Fc chimera preparations produced at R&D Systems have been shown to bind both B7-1 and B7-2 with high affinity and to inhibit CD28 signalling competitively.
Product Datasheets
Citation for Mouse CTLA-4 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Patterns of Expression of Vaginal T-Cell Activation Markers during Estrogen-Maintained Vaginal Candidiasis.
Authors: Al-Sadeq A, Hamad M, Abu-Elteen K
Allergy Asthma Clin Immunol, 2008-12-15;4(4):157-63.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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